An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging



Ingham, J, Pilling, MJ, Craig, T, Siggel-King, MRF, Smith, CI ORCID: 0000-0001-6878-0697, Gardner, P, Varro, A, Pritchard, DM ORCID: 0000-0001-7971-3561, Barrett, SD ORCID: 0000-0003-2960-3334, Martin, DS
et al (show 9 more authors) (2018) An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging. Biomedical Physics & Engineering Express, 4 (2).

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Abstract

A single human oesophageal adenocarcinoma cell (OE33) has been imaged using aperture infrared scanning near-field optical microscopy (IR-SNOM) in transmission and reflection and also by Fourier-transform infrared (FTIR) microspectroscopy in transmission only. This work presents the first images obtained in both transmission and reflection of the same specimen using the aperture IR-SNOM technique. The results have been used to compare the two SNOM modes and also the two techniques, which have complementary capabilities. The SNOM technique necessitates a very stable source and a careful choice of wavelengths, since it is too slow to yield images at the thousands of wavelengths obtained with FTIR. However the SNOM technique is not diffraction limited and with careful fabrication of tips can yield images with high spatial resolution. There is no significant correlation between the SNOM images obtained in transmission and reflection and the correlations between images obtained at different wavelengths vary with the different imaging modes. These results are attributed to the strong dependence of the evanescent wave on both the wavelength and the distance between the tip and the source of the signal within the sample. While both transmission and reflection SNOM images show some correlation with topography this is not a dominant effect. These results indicate that with suitable calibration a combination of reflection and transmission aperture IR-SNOM measurements has the potential to reveal information on the depth distribution of the chemical structure of a specimen.

Item Type: Article
Additional Information: journal_title: Biomedical Physics & Engineering Express article_type: paper article_title: An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging copyright_information: © 2018 IOP Publishing Ltd license_information: cc-by Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. date_received: 2017-08-09 date_accepted: 2017-12-12 date_epub: 2018-01-18
Uncontrolled Keywords: scanning near-field optical microscopy, Fourier-transform infrared microspectroscopy, free electron laser, adenocarcinoma cell, biomedical imaging
Depositing User: Symplectic Admin
Date Deposited: 23 Mar 2018 08:51
Last Modified: 09 Jan 2021 09:45
DOI: 10.1088/2057-1976/aaa0de
Open Access URL: http://10.0.4.64/2057-1976/aaa0de
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3019352