Analysis of snake venom metalloproteinases from Myanmar Russell's viper transcriptome

Yee, Khin Than, Tongsima, Sissades, Vasieva, Olga, Ngamphiw, Chumpol, Wilantho, Alisa, Wilkinson, Mark C, Somparn, Poorichya, Pisitkun, Trairak ORCID: 0000-0001-6677-2271 and Rojnuckarin, Ponlapat (2018) Analysis of snake venom metalloproteinases from Myanmar Russell's viper transcriptome. TOXICON, 146. pp. 31-41.

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Abstract

Snake venom metalloproteinases (SVMPs) are the key enzymes in Russell's viper (RV) venom which target all important components of haemostasis, such as clotting factors, platelets, endothelial cells and basement membrane. The structural diversity of SVMPs contributes to the broad spectrum of biological activities. The aim of the study was to investigate the SVMP transcript profile to gain better insights into the characteristic clinical manifestations of the Myanmar Russell's viper (MRV) bites that distinguish it from the RVs of other habitats. Next generation sequencing (RNA-Seq) of mRNA from MRV venom glands (2 males and 1 female) was performed on an Illumina HiSeq2000 platform and then de novo assembled using Trinity software. A total of 59 SVMP contigs were annotated through a Blastn search against the serpent nucleotide database from NCBI. Among them, disintegrins were the most abundant transcripts (75%) followed by the P-III class SVMPs (25%). The P-II SVMPs were scarce (0.002%), while no P-I SVMPs were detectable in the transcriptome. For detailed structural analysis, contigs were conceptually translated and compared with amino acid sequences from other RVs and other vipers using Clustal Omega. The RTS-disintegrin (jerdostatin homolog) was the most abundant among transcripts corresponding to 5 disintegrin isoforms. From 10 isoforms of SVMPs, RVV-X, and Vipera lebetina apoptosis-inducing protease (VLAIP) homolog, hereby termed Daboia siamensis AIP (DSAIP), were found to be highly expressed. Venom protein analysis using SDS-PAGE followed by mass spectrometry revealed that the disintegrin was scarce, while the latter two SVMPs were abundant. These two proteins can contribute to severe clinical manifestations caused by MRV envenomation.

Item Type:	Article
Uncontrolled Keywords:	Snake venom metalloproteinases, Next generation sequencing, Myanmar Russell's viper
Depositing User:	Symplectic Admin
Date Deposited:	04 Apr 2018 06:55
Last Modified:	19 Jan 2023 06:36
DOI:	10.1016/j.toxicon.2018.03.005
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3019742