Norovirus evolution : understanding and characterising the emergence of novel strains in the population


Kelly, D (2018) Norovirus evolution : understanding and characterising the emergence of novel strains in the population. PhD thesis, University of Liverpool.

[thumbnail of 200754144_September2017.pdf] Text
200754144_September2017.pdf - Unspecified
Download (27MB)

Abstract

Human noroviruses (HuNoVs) are distributed globally, affect all age groups and place a significant burden upon health services. The diversity of this RNA virus is thought to play a significant role in the persistence of HuNoVs as the main cause of non-bacterial gastroenteritis globally. Molecular diagnostics have been critical for understanding the epidemiology of outbreaks and sporadic cases, and to design and implement effective intervention strategies and disease control measures. Immunocompromised individuals are widely considered to be a reservoir for epidemic variants of HuNoV and whilst there are studies investigating the emergence of novel strains in an immunocompetent general population, reports at the the individual level are scarce. Three separate methodologies were developed to characterise HuNoV persistence in acute convalescent and chronic infection. First, a standardised quantification method to accurately quantify the most prevalent HuNoV genogroup. Second, a PGM-MB capture method to select HuNoV prior to massively parallel sequencing (MPS). Third, an assay to measure host specific coproantibody responses to three epidemic variants from different epochs. Quantification of longitudinal samples ... (continues)

Item Type: Thesis (PhD)
Divisions: Faculty of Health and Life Sciences > Faculty of Health and Life Sciences
Depositing User: Symplectic Admin
Date Deposited: 28 Aug 2018 09:25
Last Modified: 24 Apr 2025 06:52
DOI: 10.17638/03021059
Supervisors:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3021059
Altmetric
CORE (COnnecting REpositories)