Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells

Chowdhury, Neelika Roy, Hopp, Isabel, Zilm, Peter, Murray, Patricia ORCID: 0000-0003-1316-148X and Vasilev, Krasimir (2018) Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells. RSC ADVANCES, 8 (36). pp. 20334-20340.

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Official URL: http://dx.doi.org/10.1039/c8ra02145g

Abstract

In this paper, we interrogate the influence of silver nanoparticle (AgNPs)-based model surfaces on mouse kidney-derived stem cells (mKSCs) differentiation. The widespread use of silver in biomedical and consumer products requires understanding of this element's effect on kidney cells. Moreover, the potential for using stem cells in drug discovery require methods to direct their differentiation to specialized cells. Hence, we generated coated model substrates containing different concentrations of surface immobilized AgNPs, and used them to evaluate properties and functions of mKSCs. Initially, mKSCs exhibited reduced viability on higher silver containing surfaces. However, longer culture periods assisted mKSCs to recover. Greater degree of cell spreading and arborization led by AgNPs, suggest podocyte differentiation. Proximal tubule cell marker's expression revealed differentiation to the specific lineage. Although the exact mechanism underpinning these findings require significant future efforts, this study demonstrate silver's capacity to stimulate mKSC differentiation, which may provide opportunities for drug screenings.

Item Type:	Article
Uncontrolled Keywords:	Bioengineering, Regenerative Medicine, Kidney Disease, Biotechnology, Stem Cell Research, Nanotechnology, 1 Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Renal and urogenital
Depositing User:	Symplectic Admin
Date Deposited:	11 Jun 2018 13:17
Last Modified:	15 Mar 2024 01:11
DOI:	10.1039/c8ra02145g
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3022432