Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells



Hammond, Dean E ORCID: 0000-0002-6326-8739, Kumar, J Dinesh, Raymond, Lorna, Simpson, Deborah M ORCID: 0000-0002-3962-4895, Beynon, Robert J ORCID: 0000-0003-0857-495X, Dockray, Graham J and Varro, Andrea
(2018) Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells. MOLECULAR & CELLULAR PROTEOMICS, 17 (9). 1837 - 1849.

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Abstract

Analysis of secretomes critically underpins the capacity to understand the mechanisms determining interactions between cells and between cells and their environment. In the context of cancer cell micro-environments, the relevant interactions are recognized to be an important determinant of tumor progression. Global proteomic analyses of secretomes are often performed at a single time point and frequently identify both classical secreted proteins (possessing an N-terminal signal sequence), as well as many intracellular proteins, the release of which is of uncertain biological significance. Here, we describe a mass spectrometry-based method for stable isotope dynamic labeling of secretomes (SIDLS) that, by dynamic SILAC, discriminates the secretion kinetics of classical secretory proteins and intracellular proteins released from cancer and stromal cells in culture. SIDLS is a robust classifier of the different cellular origins of proteins within the secretome and should be broadly applicable to nonproliferating cells and cells grown in short term culture.

Item Type: Article
Uncontrolled Keywords: Secretome, SILAC, Cancer biology, Exocytosis, Cell secretion, Dynamic labelling, Short term labelling
Depositing User: Symplectic Admin
Date Deposited: 29 Jun 2018 09:49
Last Modified: 12 Jan 2021 12:01
DOI: 10.1074/mcp.TIR117.000516
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3023173