Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa


Bricio-Moreno, L, Sheridan, VH ORCID: 0009-0005-7214-805X, Goodhead, I, Armstrong, S ORCID: 0000-0002-3862-1801, Wong, JKL, Waters, EM, Sarsby, J ORCID: 0000-0002-1942-6306, Panagiotou, S, Dunn, J, Chakraorty, A et al (show 6 more authors) (2018) Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa Nature Communications, 9 (1). 2635-. ISSN 2041-1723, 2041-1723

[thumbnail of Bricio-Moreno_et_al-2018-Nature_Communications.pdf] Text
Bricio-Moreno_et_al-2018-Nature_Communications.pdf - Published version
Download (1MB)

Abstract

Pseudomonas aeruginosa colonises the upper airway of cystic fibrosis (CF) patients, providing a reservoir of host-adapted genotypes that subsequently establish chronic lung infection. We previously experimentally-evolved P. aeruginosa in a murine model of respiratory tract infection and observed early-acquired mutations in pmrB, encoding the sensor kinase of a two-component system that promoted establishment and persistence of infection. Here, using proteomics, we show downregulation of proteins involved in LPS biosynthesis, antimicrobial resistance and phenazine production in pmrB mutants, and upregulation of proteins involved in adherence, lysozyme resistance and inhibition of the chloride ion channel CFTR, relative to wild-type strain LESB65. Accordingly, pmrB mutants are susceptible to antibiotic treatment but show enhanced adherence to airway epithelial cells, resistance to lysozyme treatment, and downregulate host CFTR expression. We propose that P. aeruginosa pmrB mutations in CF patients are subject to an evolutionary trade-off, leading to enhanced colonisation potential, CFTR inhibition, and resistance to host defences, but also to increased susceptibility to antibiotics.

Item Type: Article
Uncontrolled Keywords: Lung, Fimbriae, Bacterial, Epithelial Cells, Animals, Humans, Mice, Pseudomonas aeruginosa, Pseudomonas Infections, Muramidase, Bacterial Proteins, Cystic Fibrosis Transmembrane Conductance Regulator, Transcription Factors, Anti-Infective Agents, Colony Count, Microbial, Microbial Sensitivity Tests, Proteomics, Adaptation, Physiological, Bacterial Adhesion, Down-Regulation, Movement, Mutation, Principal Component Analysis, Models, Biological, Host-Pathogen Interactions, Biological Evolution, A549 Cells
Depositing User: Symplectic Admin
Date Deposited: 06 Jul 2018 10:37
Last Modified: 16 Jun 2026 07:06
DOI: 10.1038/s41467-018-04996-x
Related Websites:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3023433
Disclaimer: The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.
Altmetric
CORE (COnnecting REpositories)