Dynamic loading of electrospun yarns guides mesenchymal stem cells towards a tendon lineage

Bosworth, LA ORCID: 0000-0002-6726-4663, Rathbone, SR, Bradley, RS and Cartmell, SH (2014) Dynamic loading of electrospun yarns guides mesenchymal stem cells towards a tendon lineage JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 39. pp. 175-183. ISSN 1751-6161, 1878-0180

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Abstract

Alternative strategies are required when autograft tissue is not sufficient or available to reconstruct damaged tendons. Electrospun fibre yarns could provide such an alternative. This study investigates the seeding of human mesenchymal stem cells (hMSC) on electrospun yarns and their response when subjected to dynamic tensile loading. Cell seeded yarns sustained 3600 cycles per day for 21 days. Loaded yarns demonstrated a thickened cell layer around the scaffold׳s exterior compared to statically cultured yarns, which would suggest an increased rate of cell proliferation and/or matrix deposition, whilst maintaining a predominant uniaxial cell orientation. Tensile properties of cell-seeded yarns increased with time compared to acellular yarns. Loaded scaffolds demonstrated an up-regulation in several key tendon genes, including collagen Type I. This study demonstrates the support of hMSCs on electrospun yarns and their differentiation towards a tendon lineage when mechanically stimulated.

Item Type:	Article
Uncontrolled Keywords:	Electrospinning, Tendon, Poly(epsilon-caprolactone), Dynamic loading, Cyclical loading, Mesenchymal stem cells
Depositing User:	Symplectic Admin
Date Deposited:	13 Aug 2018 06:35
Last Modified:	16 Jun 2026 09:30
DOI:	10.1016/j.jmbbm.2014.07.009
Open Access URL:	https://www.sciencedirect.com/science/article/pii/...
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3024869
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