Nightingale, S, Michael, BD ORCID: 0000-0002-8693-8926, Fisher, M, Winston, A, Nelson, M, Taylor, S, Ustianowski, A, Ainsworth, J, Gilson, R, Haddow, L et al (show 10 more authors)
(2016)
CSF/plasma HIV-1 RNA discordance even at low levels is associated with up-regulation of host inflammatory mediators in CSF.
Cytokine, 83.
pp. 139-146.
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Abstract
Introduction HIV-1 RNA can be found at higher levels in cerebrospinal fluid (CSF) than in plasma, termed CSF/plasma discordance. The clinical significance of CSF/plasma discordance is not known and the degree of discordance considered important varies. We aimed to determine whether a panel of CSF cytokines, chemokines and associated mediators were raised in patients with CSF/plasma discordance at different levels. Methods A nested case-control study of 40 CSF samples from the PARTITION study. We used a cytometric bead array to measure CSF mediator concentrations in 19 discordant and 21 non-discordant samples matched for plasma HIV-1 RNA. Discordant samples were subdivided into ‘high discordance’ (>1log10) and ‘low discordance’ (0.5–1log10, or ultrasensitive discordance). CSF mediators significant in univariate analysis went forward to two-way unsupervised hierarchical clustering based on the patterns of relative mediator concentrations. Results In univariate analysis 19 of 21 CSF mediators were significantly higher in discordant than non-discordant samples. There were no significant differences between samples with high versus low discordance. The samples grouped into two clusters which corresponded to CSF/plasma discordance (p < 0.0001). In cluster one all mediators had relatively high abundance; this included 18 discordant samples and three non-discordant samples. In cluster two all mediators had relatively low abundance; this included 18 non-discordant samples and one non-discordant sample with ultrasensitive discordance only. Conclusions CSF/plasma discordance is associated with potentially damaging neuroinflammatory process. Patients with discordance at lower levels (ie. 0.5–1log10) should also be investigated as mediator profiles were similar to those with discordance >1log10. Sensitive testing may have a role to determine whether ultrasensitive discordance is present in those with low level CSF escape.
Item Type: | Article |
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Uncontrolled Keywords: | HIV, Cerebrospinal fluid, CSF escape, Sanctuary site, Inflammation |
Depositing User: | Symplectic Admin |
Date Deposited: | 17 Aug 2018 14:24 |
Last Modified: | 19 Jan 2023 01:28 |
DOI: | 10.1016/j.cyto.2016.04.004 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3025152 |
Available Versions of this Item
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CSF/plasma HIV-1 RNA discordance even at low levels is associated with up-regulation of host inflammatory mediators in CSF. (deposited 25 May 2016 09:15)
- CSF/plasma HIV-1 RNA discordance even at low levels is associated with up-regulation of host inflammatory mediators in CSF. (deposited 17 Aug 2018 14:24) [Currently Displayed]