Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes



Comenge, Joan, Sharkey, Jack, Fragueiro, Oihane, Wilm, Bettina, Brust, Mathias ORCID: 0000-0001-6301-7123, Murray, Patricia ORCID: 0000-0003-1316-148X, Lévy, Raphaël ORCID: 0000-0001-5728-0531 and Plagge, Antonius
(2017) Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes.

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Abstract

ABSTRACT Understanding the fate of exogenous cells after implantation is important for clinical applications. Preclinical studies allow imaging of cell location and survival. Labelling with nanoparticles enables high sensitivity detection, but cell division and cell death cause signal dilution and false positives. By contrast, genetic reporter signals are amplified by cell division. Here we characterise lentivirus-based bi-cistronic reporter gene vectors and silica-coated gold nanorods (GNRs) as synergistic tools for cell labelling and tracking. Co-expression of the bioluminescence reporter luciferase and the optoacoustic reporter near-infrared fluorescent protein iRFP720 enabled cell tracking over time in mice. Multispectral optoacoustic tomography (MSOT) showed immediate biodistribution of GNR-labelled cells after intracardiac injection and successive clearance of GNRs (day 1-15) with high resolution, while optoacoustic iRFP720 detection indicated tumour growth (day 10-40). This multimodal cell tracking approach could be applied widely for cancer and regenerative medicine research to monitor short- and long-term biodistribution, tumour formation and metastasis.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 14 Sep 2018 10:37
Last Modified: 25 Feb 2020 09:22
DOI: 10.1101/199836
Open Access URL: https://elifesciences.org/articles/33140
URI: http://livrepository.liverpool.ac.uk/id/eprint/3026215