1H NMR metabolomics identifies underlying inflammatory pathology in osteoarthritis and rheumatoid arthritis synovial joints



Anderson, James ORCID: 0000-0003-0489-7997, Chokesuwattanaskul, Susama, Phelan, Marie, Welting, Tim, Lian, Lu Yun, Peffers, Mandy ORCID: 0000-0001-6979-0440 and Wright, HL
(2018) 1H NMR metabolomics identifies underlying inflammatory pathology in osteoarthritis and rheumatoid arthritis synovial joints. Journal of Proteome Research, 17 (11). 3780 - 3790.

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Abstract

Despite osteoarthritis (OA) and rheumatoid arthritis (RA) being typically age-related, their underlying aetiologies are markedly different. We used 1H Nuclear Magnetic Resonance (NMR) spectroscopy to identify differences in metabolite profiles in low volumes of OA and RA synovial fluid (SF). SF was aspirated from knee joints of 10 OA and 14 RA patients. 100µL SF was analysed using a 700 MHz Avance IIIHD Bruker NMR spectrometer with a TCI cryoprobe. Spectra were analysed by Chenomx, Bruker TopSpin and AMIX software. Statistical analysis was undertaken using Metaboanalyst®. 50 metabolites were annotated, including amino acids, saccharides, nucleotides and soluble lipids. Discriminant analysis identified group separation between OA and RA cohorts, with 32 metabolites significantly different between OA and RA SF (False Discovery Rate (FDR)<0.05). Metabolites of glycolysis and the tricarboxylic acid cycle were lower in RA compared to OA; these results concur with higher levels of inflammation, synovial proliferation and hypoxia found in RA compared to OA. Elevated taurine in OA may indicate increased subchondral bone sclerosis. We demonstrate that quantifiable differences in metabolite abundance can be measured in low volumes of SF by 1H NMR spectroscopy, which may be clinically useful to aid diagnosis and improve understanding of disease pathogenesis.

Item Type: Article
Uncontrolled Keywords: peptides and proteins, carbohydrates, metabolism, monomerss, glycolysis
Depositing User: Symplectic Admin
Date Deposited: 20 Sep 2018 10:40
Last Modified: 13 Jan 2021 16:11
DOI: 10.1021/acs.jproteome.8b00455
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3026500