Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

Smith, Caroline I ORCID: 0000-0001-6878-0697, Siggel-King, Michele RF, Ingham, James, Harrison, Paul, Martin, David S, Varro, Andrea, Pritchard, D Mark ORCID: 0000-0001-7971-3561, Surman, Mark, Barrett, Steve ORCID: 0000-0003-2960-3334 and Weightman, Peter ORCID: 0000-0002-0907-3930 (2018) Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell. ANALYST, 143 (24). pp. 5912-5917.

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Abstract

This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm-1, 1651 cm-1, 1539 cm-1 and 1242 cm-1. In addition to the 1651 cm-1 image, three other images were acquired within the Amide I band (1689 cm-1, 1675 cm-1 and 1626 cm-1) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.

Item Type:	Article
Uncontrolled Keywords:	Cell Line, Tumor, Epithelial Cells, Humans, Adenocarcinoma, Microscopy, Lasers, Semiconductor
Depositing User:	Symplectic Admin
Date Deposited:	24 Jan 2019 08:54
Last Modified:	19 Jan 2023 01:16
DOI:	10.1039/c8an01183d
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3026729