Characterisation of potential therapeutic molecules for neuroblastoma using chick chorioallantoic membrane xenograft model

Swadi, Rasha ORCID: 0000-0002-0758-9247 (2018) Characterisation of potential therapeutic molecules for neuroblastoma using chick chorioallantoic membrane xenograft model PhD thesis, University of Liverpool.

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Abstract

Neuroblastoma is a paediatric cancer derived from the sympathoadrenal cell lineage that commonly presents as a high-risk metastatic disease with a poor prognosis. Currently, differentiation therapy with retinoic acid is an effective therapeutic option used in the clinic, however not all tumours respond. New therapeutic agents that can control not only tumour growth but also metastasis are urgently needed and drugs that promote differentiation are particularly appropriate for neuroblastoma treatment. Preclinical models are required to aid the development of novel therapeutics for this challenging childhood malignancy, however current systems are complex and inherently costly. Here we employ a 3Rs-compliant cost-effective preclinical chick embryo model to act as a tool for neuroblastoma therapeutic research. Neuroblastoma cell lines were engrafted on the chorioallantoic membrane (CAM) of chick embryos and allowed to form tumours over a 7 day period. If cells were preconditioned in hypoxia they metastasised into the chick embryo. First, we validated the chick embryo model using retinoic acid and then investigated the potential of CDK inhibitors on neuroblastoma cell differentiation, tumour progression and metastasis. ... (continues)

Item Type:	Thesis (PhD)
Divisions:	Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences > School of Medicine
Depositing User:	Symplectic Admin
Date Deposited:	28 Nov 2018 12:57
Last Modified:	31 Oct 2024 07:01
DOI:	10.17638/03026946
Supervisors:	Moss, Diana Campbell, Barry ORCID: 0000-0002-7407-012X Kalirai, Helen ORCID: 0000-0002-4440-2576 Coupland, Sarah ORCID: 0000-0002-1464-2069
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3026946
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