SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression

Biddlestone, John, Batie, Michael ORCID: 0000-0002-7508-6641, Bandarra, Daniel, Munoz, Ivan and Rocha, Sonia (2018) SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression. BIOCHEMICAL JOURNAL, 475 (12). pp. 2073-2090.

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Abstract

The SIN3A-HDAC (histone deacetylase) complex is a master transcriptional repressor, required for development but often deregulated in disease. Here, we report that the recently identified new component of this complex, SINHCAF (SIN3A and HDAC-associated factor)/FAM60A (family of homology 60A), links the SIN3A-HDAC co-repressor complex function to the hypoxia response. We show that SINHCAF specifically represses HIF-2α mRNA and protein expression, via its interaction with the transcription factor SP1 (specificity protein 1) and recruitment of HDAC1 to the HIF-2α promoter. SINHCAF control over HIF-2α results in functional cellular changes in <i>in vitro</i> angiogenesis and viability. Our analysis reveals an unexpected link between SINHCAF and the regulation of the hypoxia response.

Item Type:	Article
Uncontrolled Keywords:	Hela Cells, Humans, DNA-Binding Proteins, Repressor Proteins, RNA, Messenger, Gene Expression Regulation, Basic Helix-Loop-Helix Transcription Factors, Promoter Regions, Genetic, Sin3 Histone Deacetylase and Corepressor Complex, Histone Deacetylase 1, A549 Cells
Depositing User:	Symplectic Admin
Date Deposited:	04 Oct 2018 08:18
Last Modified:	19 Oct 2023 09:18
DOI:	10.1042/BCJ20170945
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3027089