A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat

Pijanka, Jacek Klaudiusz, Abass, Ahmed, Sorensen, Thomas, Elsheikh, Ahmed and Boote, Craig (2013) A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 46 (5). pp. 1481-1489.

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Abstract

<jats:p>A quantitative map of collagen fibril orientation across the human eyeball coat, including both the cornea and the sclera, has been obtained using a combination of synchrotron wide-angle X-ray scattering (WAXS) and three-dimensional point mapping. A macromolecular crystallography beamline, in a custom-modified fibre diffraction setup, was used to record the 1.6 nm intermolecular equatorial reflection from fibrillar collagen at 0.5 mm spatial resolution across a flat-mounted human eyeball coat. Fibril orientation, derived as an average measure of the tissue thickness, was quantified by extraction of the azimuthal distribution of WAXS scatter intensity. Vector plots of preferential fibre orientation were remapped onto an idealized eyeball surface using a custom-built numerical algorithm, to obtain a three-dimensional representation of the collagen fibril architecture.</jats:p>

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	3402 Inorganic Chemistry, 34 Chemical Sciences, 3406 Physical Chemistry, 51 Physical Sciences, 5104 Condensed Matter Physics, Bioengineering
Depositing User:	Symplectic Admin
Date Deposited:	15 Nov 2018 15:14
Last Modified:	20 Jun 2024 17:28
DOI:	10.1107/S0021889813022358
Open Access URL:	http://scripts.iucr.org/cgi-bin/paper?S00218898130...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3028891

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• A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat. (deposited 15 Jun 2017 10:57)
  • A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat. (deposited 15 Nov 2018 15:14) [Currently Displayed]