In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells



Ashraf, Sumaira, Taylor, Arthur ORCID: 0000-0003-2028-6694, Sharkey, Jack ORCID: 0000-0002-0516-7622, Barrow, Michael, Murray, Patricia, Wilm, Bettina ORCID: 0000-0002-9245-993X, Poptani, Harish, Rosseinsky, Matthew ORCID: 0000-0002-1910-2483, Adams, Dave and Levy, Raphael ORCID: 0000-0001-5728-0531
(2018) In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells. Nanoscale Advances.

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Abstract

Nanoparticle contrast agents are useful tools to label stem cells and monitor the in vivo bio-distribution of labeled cells in pre-clinical models of disease. In this context, understanding the in vivo fate of the particles after injection of labelled cells is important for their eventual clinical use as well as for the interpretation of imaging results. We examined how the formulation of superparamagnetic iron oxide nanoparticles (SPIONs) impacts the labelling efficiency, magnetic characteristics and fate of the particles by comparing individual SPIONs with polyelectrolyte multilayer capsules containing SPIONs. At low labelling concentration, encapsulated SPIONs served as an efficient labelling agent for stem cells. The bio-distribution after intra-cardiac injection of labelled cells was monitored longitudinally by MRI and as an endpoint by inductively coupled plasma-optical emission spectrometry. The results suggest that, after being released from labelled cells after cell death, both formulations of particles are initially stored in liver and spleen and are not completely cleared from these organs 2 weeks post-injection.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 18 Dec 2018 09:20
Last Modified: 20 Nov 2019 04:10
DOI: 10.1101/366518
Open Access URL: http://dx.doi.org/10.1039/C8NA00098K
URI: http://livrepository.liverpool.ac.uk/id/eprint/3030187
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