Kharaz, Yalda A, Tew, Simon R ORCID: 0000-0002-1213-5418, Peffers, Mandy ORCID: 0000-0001-6979-0440, Canty-Laird, Elizabeth G ORCID: 0000-0001-5041-1316 and Comerford, Eithne ORCID: 0000-0002-5244-6042
(2016)
Proteomic differences between native and tissue-engineered tendon and ligament.
PROTEOMICS, 16 (10).
pp. 1547-1556.
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Abstract
Tendons and ligaments (T/Ls) play key roles in the musculoskeletal system, but they are susceptible to traumatic or age-related rupture, leading to severe morbidity as well as increased susceptibility to degenerative joint diseases such as osteoarthritis. Tissue engineering represents an attractive therapeutic approach to treating T/L injury but it is hampered by our poor understanding of the defining characteristics of the two tissues. The present study aimed to determine differences in the proteomic profile between native T/Ls and tissue engineered (TE) T/L constructs. The canine long digital extensor tendon and anterior cruciate ligament were analyzed along with 3D TE fibrin-based constructs created from their cells. Native tendon and ligament differed in their content of key structural proteins, with the ligament being more abundant in fibrocartilaginous proteins. 3D T/L TE constructs contained less extracellular matrix (ECM) proteins and had a greater proportion of cellular-associated proteins than native tissue, corresponding to their low collagen and high DNA content. Constructs were able to recapitulate native T/L tissue characteristics particularly with regard to ECM proteins. However, 3D T/L TE constructs had similar ECM and cellular protein compositions indicating that cell source may not be an important factor for T/L tissue engineering.
Item Type: | Article |
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Uncontrolled Keywords: | 3D tissue engineered ligament construct, 3D tissue engineered tendon constructs, Ligament, Proteomics, Tendon, Technology |
Depositing User: | Symplectic Admin |
Date Deposited: | 18 Jan 2019 14:25 |
Last Modified: | 19 Jan 2023 01:08 |
DOI: | 10.1002/pmic.201500459 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3030733 |
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Proteomic differences between native and tissue-engineered tendon and ligament. (deposited 26 Apr 2016 15:49)
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