Redox responses are preserved across muscle fibres with differential susceptibility to aging



Smith, Neil T, Soriano-Arroquia, Ana, Goljanek-Whysall, Katarzyna, Jackson, Malcolm J ORCID: 0000-0003-3683-8297 and McDonagh, Brian
(2018) Redox responses are preserved across muscle fibres with differential susceptibility to aging. Journal of Proteomics, 177. 112 - 123.

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Abstract

Age-related loss of muscle mass and function is associated with increased frailty and loss of independence. The mechanisms underlying the susceptibility of different muscle types to age-related atrophy are not fully understood. Reactive oxygen species (ROS) are recognised as important signalling molecules in healthy muscle and redox sensitive proteins can respond to intracellular changes in ROS concentrations modifying reactive thiol groups on Cysteine (Cys) residues. Conserved Cys residues tend to occur in functionally important locations and can have a direct impact on protein function through modifications at the active site or determining protein conformation. The aim of this work was to determine age-related changes in the redox proteome of two metabolically distinct murine skeletal muscles, the quadriceps a predominantly glycolytic muscle and the soleus which contains a higher proportion of mitochondria. To examine the effects of aging on the global proteome and the oxidation state of individual redox sensitive Cys residues, we employed a label free proteomics approach including a differential labelling of reduced and reversibly oxidised Cys residues. Our results indicate the proteomic response to aging is dependent on muscle type but redox changes that occur primarily in metabolic and cytoskeletal proteins are generally preserved between metabolically distinct tissues.

Item Type: Article
Uncontrolled Keywords: Skeletal muscle, Aging, Redox proteomics, Cysteine oxidation, Sarcopenia, skNAC
Depositing User: Symplectic Admin
Date Deposited: 18 Jan 2019 14:28
Last Modified: 12 Nov 2021 02:10
DOI: 10.1016/j.jprot.2018.02.015
Open Access URL: https://www.sciencedirect.com/science/article/pii/...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3030737