Chen, Guangyu E, Canniffe, Daniel P ORCID: 0000-0002-5022-0437, Barnett, Samuel FH, Hollingshead, Sarah, Brindley, Amanda A, Vasilev, Cvetelin, Bryant, Donald A and Hunter, C Neil
(2018)
Complete enzyme set for chlorophyll biosynthesis in Escherichia coli.
SCIENCE ADVANCES, 4 (1).
eaaq1407-.
ISSN 2375-2548, 2375-2548
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Complete enzyme set for chlorophyll biosynthesis in Escherichia coli.pdf - Published version Download (1MB) |
Abstract
Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg-protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in <i>Escherichia coli</i>. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning <i>E. coli</i> cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.
Item Type: | Article |
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Uncontrolled Keywords: | Escherichia coli, Protoporphyrins, Recombinant Proteins, Biosynthetic Pathways, Metabolic Engineering |
Depositing User: | Symplectic Admin |
Date Deposited: | 17 Jan 2019 09:24 |
Last Modified: | 07 Dec 2024 19:04 |
DOI: | 10.1126/sciadv.aaq1407 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3031304 |