Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

Song, Linlin, Han, Ximeng, Jia, Cunyu, Zhang, Xin, Jiao, Yunjie, Du, Taofeng, Xiao, Shuqi, Hiscox, Julian A ORCID: 0000-0002-6582-0275, Zhou, En-Min and Mu, Yang (2018) Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway. VIROLOGY JOURNAL, 15 (1). 169-.

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Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway.pdf - Published version
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Official URL: http://dx.doi.org/10.1186/s12985-018-1081-9

Abstract

Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at G2/M phase. This arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected cells and the activity reduction was a result of Cdc2 Tyr15 phosphorylation and the accumulation of Cdc2 and cyclinB1 in the nucleus. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25C, but also increase of p21 and 14-3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. PRRSV infection also activated Chk1. Our data suggest PRRSV infection induces G2/M arrest via various molecular regulatory mechanisms. These results provide a new insights for PRRSV pathogenesis.

Item Type:	Article
Uncontrolled Keywords:	Porcine reproductive and respiratory syndrome virus, G2, M arrest, Cdc2-cyclinB1 complex, Cdc25C, p53
Depositing User:	Symplectic Admin
Date Deposited:	25 Jan 2019 09:42
Last Modified:	19 Jan 2023 01:06
DOI:	10.1186/s12985-018-1081-9
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3031735