Oral iron exacerbates colitis and influences the intestinal microbiome


Mahalhal, Awad, Williams, Jonathan, Johnson, Sophie, Ellaby, Nicholas, Duckworth, Carrie ORCID: 0000-0001-7971-3561, Burkitt, Michael, Liu, Xuan, Hold, Georgina ORCID: 0000-0001-7573-3397, Campbell, Barry ORCID: 0000-0002-7407-012X, Pritchard, Mark ORCID: 0000-0001-7971-3561 et al (show 1 more authors) (2018) Oral iron exacerbates colitis and influences the intestinal microbiome 385997-. ISSN 2692-8205

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Abstract

Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage. The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD. Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA. Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis. DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p<0.01. Faecal calprotectin levels were significantly increased in the presence of colitis in those consuming 100 ppm iron at day 8 (5.94-fold) versus day-10 group (4.14-fold) (p<0.05), and for the 400 ppm day-8 group (8.17-fold) versus day-10 group (4.44-fold) (p<0.001). In the presence of colitis, dietary iron at 400 ppm resulted in a significant reduction in faecal abundance of Firmicutes and Bacteroidetes, and increase of Proteobacteria, changes which were not observed with lower dietary intake of iron at 100 ppm. Overall, altering dietary iron intake exacerbated DSS-induced colitis; increasing the iron content of the diet also led to changes in intestinal bacteria diversity and composition after colitis was induced with DSS.

Item Type: Article
Uncontrolled Keywords: 3107 Microbiology, 31 Biological Sciences, Autoimmune Disease, Dietary Supplements, Complementary and Integrative Health, Digestive Diseases, Microbiome, Crohn's Disease, Inflammatory Bowel Disease, Nutrition, 2.1 Biological and endogenous factors, Oral and gastrointestinal
Depositing User: Symplectic Admin
Date Deposited: 30 Jan 2019 13:22
Last Modified: 29 Jan 2026 17:58
DOI: 10.1101/385997
Open Access URL: https://journals.plos.org/plosone/article?id=10.13...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3032014
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