Transdermal Measurement of Glomerular Filtration Rate in Mice.



Scarfe, Lauren, Schock-Kusch, Daniel, Ressel, Lorenzo ORCID: 0000-0002-6614-1223, Friedemann, Jochen, Shulhevich, Yury, Murray, Patricia, Wilm, Bettina ORCID: 0000-0002-9245-993X and de Caestecker, Mark
(2018) Transdermal Measurement of Glomerular Filtration Rate in Mice. Journal of visualized experiments : JoVE, 140.

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Abstract

Transdermal analysis of glomerular filtration rate (GFR) is an established technique that is used to assess renal function in mouse and rat models of acute kidney injury and chronic kidney disease. The measurement system consists of a miniaturized fluorescence detector that is directly attached to the skin on the back of conscious, freely moving animals, and measures the excretion kinetics of the exogenous GFR tracer, fluorescein-isothiocyanate (FITC) conjugated sinistrin (an inulin analog). This system has been described in detail in rats. However, because of their smaller size, measurement of transcutaneous GFR in mice presents additional technical challenges. In this paper we therefore provide the first detailed practical guide to the use of transdermal GFR monitors in mice based on the combined experience of three different investigators who have been performing this assay in mice over a number of years.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 01 Feb 2019 16:07
Last Modified: 07 Jul 2021 11:23
DOI: 10.3791/58520
Open Access URL: https://www.jove.com/video/58520/transdermal-measu...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3032137