Sensitivity and specificity of microRNA-122 for liver disease in dogs

Oosthuyzen, W, Ten Berg, PWL, Francis, B ORCID: 0000-0002-2130-5976, Campbell, S, Macklin, V, Milne, E, Gow, AG, Fisher, C, Mellanby, RJ and Dear, JW
(2018) Sensitivity and specificity of microRNA-122 for liver disease in dogs. JOURNAL OF VETERINARY INTERNAL MEDICINE, 32 (5). pp. 1637-1644.

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<h4>Background</h4>Current tests for diagnosing liver disease in dogs are sub-optimal. MicroRNA-122 (miR-122) is a sensitive and specific biomarker of liver injury in humans and rodents. Circulating miR-122 could have utility in identifying dogs with liver disease.<h4>Objective</h4>Establish the reference interval for miR-122 in healthy dogs and determine performance in a range of dog breeds with liver disease and control animals with non-liver disease.<h4>Animals</h4>Stored serum from 120 healthy dogs, 100 dogs with non-liver diseases, and 30 dogs with histologically confirmed liver disease was analyzed.<h4>Methods</h4>Retrospective study. Medical records of dogs with liver disease, non-liver disease and healthy dogs were reviewed. Serum miR-122 concentrations were measured by PCR and compared with the characteristics of the dogs and their conventional clinical measurements.<h4>Results</h4>In healthy dogs the 2.5th, 50th, and 97.5th quartiles of miR-122 were 110 (90% CI 80-114), 594 (505-682), and 3312 (2925-5144) copies/μL, respectively. There was no difference between healthy dogs and dogs with non-liver disease (median ± IQR: healthy dogs 609 [327-1014] copies/μL; non-liver disease 607 [300-1351] copies/μL). miR-122 was higher in dogs with liver disease (11 332 [4418-20 520] copies/μL, P < .001 compared to healthy dogs). miR-122 identified dogs with liver disease with high accuracy (receiver operating characteristic area under curve for comparison with healthy dogs: 0.93 [95% CI 0.86-0.99]). The upper limit of normal for healthy dogs (3312 copies/μL) had a sensitivity of 77% and specificity of 97% for identifying liver disease.<h4>Conclusion and clinical importance</h4>Liver disease can be sensitively and specifically diagnosed in dogs by measurement of miR-122.

Item Type: Article
Uncontrolled Keywords: biomarker, canine, hepatic, microRNA
Depositing User: Symplectic Admin
Date Deposited: 01 Feb 2019 16:26
Last Modified: 19 Jan 2023 01:05
DOI: 10.1111/jvim.15250
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