van Vliet, Erwin, Kuehnl, Jochen, Goebel, Carsten, Martinozzi-Teissier, Silvia, Alepee, Nathalie, Ashikaga, Takao, Bloemeke, Brunhilde, Del Bufalo, Aurelia, Cluzel, Magalie, Corsini, Emanuela et al (show 12 more authors)
(2018)
State-of-the-Art and New Options to Assess T Cell Activation by Skin Sensitizers: Cosmetics Europe Workshop.
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION, 35 (2).
pp. 179-192.
Abstract
Significant progress has been made in the development and validation of non-animal test methods for skin sensitization assessment. At present, three of the four key events of the Adverse Outcome Pathway (AOP) are assessable by OECD-accepted in vitro methods. The fourth key event describes the immunological response in the draining lymph node where activated dendritic cells present major histocompatibility complex-bound chemically modified peptides to naive T cells, thereby priming the proliferation of antigen-specific T cells. Despite substantial efforts, modelling and assessing this adaptive immune response to sensitizers with in vitro T cell assays still represents a challenge. The Cosmetics Europe Skin Tolerance Task Force organized a workshop, bringing together academic researchers, method developers, industry representatives and regulatory stakeholders to review the scientific status of T cell-based assays, foster a mutual scientific understanding and conceive new options to assess T cell activation. Participants agreed that current T cell assays have come a long way in predicting immunogenicity, but that further investment and collaboration is required to simplify assays, optimize their sensitivity, better define human donor-to-donor variability and evaluate their value to predict sensitizer potency. Furthermore, the potential role of T cell assays in AOP-based testing strategies and subsequent safety assessment concepts for cosmetic ingredients was discussed. It was agreed that it is currently difficult to anticipate uses of T cell assay data for safety assessment and concluded that experience from case studies on real-life risk assessment scenarios is needed to further consider the usefulness of assessing the fourth AOP key event.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | skin sensitization, adverse outcome pathway, key events, T cell assays, safety assessment |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 04 Feb 2019 11:08 |
| Last Modified: | 19 Jan 2023 01:05 |
| DOI: | 10.14573/altex.1709011 |
| Open Access URL: | https://www.altex.org/index.php/altex/article/view... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3032234 |
Dimensions
Dimensions
