A Strain of an Emerging Indian <i>Xanthomonas oryzae</i> pv. oryzae Pathotype Defeats the Rice Bacterial Blight Resistance Gene <i>xa13</i> Without Inducing a Clade III <i>SWEET</i> Gene and Is Nearly Identical to a Recent Thai Isolate


Carpenter, Sara CD, Mishra, Prashant, Ghoshal, Chandrika, Dash, Prasanta K, Wang, Li, Midha, Samriti, Laha, Gouri S, Lore, Jagjeet S, Kositratana, Wichai, Singh, Nagendra K et al (show 6 more authors) (2018) A Strain of an Emerging Indian <i>Xanthomonas oryzae</i> pv. oryzae Pathotype Defeats the Rice Bacterial Blight Resistance Gene <i>xa13</i> Without Inducing a Clade III <i>SWEET</i> Gene and Is Nearly Identical to a Recent Thai Isolate. FRONTIERS IN MICROBIOLOGY, 9 (NOV). 2703-.

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Abstract

The rice bacterial blight pathogen <i>Xanthomonas oryzae</i> pv. oryzae (<i>Xoo</i>) injects transcription activator-like effectors (TALEs) that bind and activate host "susceptibility" (<i>S</i>) genes important for disease. Clade III <i>SWEET</i> genes are major S genes for bacterial blight. The resistance genes <i>xa5</i>, which reduces TALE activity generally, and <i>xa13</i>, a <i>SWEET11</i> allele not recognized by the cognate TALE, have been effectively deployed. However, strains that defeat both resistance genes individually were recently reported in India and Thailand. To gain insight into the mechanism(s), we completely sequenced the genome of one such strain from each country and examined the encoded TALEs. Strikingly, the two strains are clones, sharing nearly identical TALE repertoires, including a TALE known to activate <i>SWEET11</i> strongly enough to be effective even when diminished by <i>xa5</i>. We next investigated <i>SWEET</i> gene induction by the Indian strain. The Indian strain induced no clade III <i>SWEET</i> in plants harboring <i>xa13</i>, indicating a pathogen adaptation that relieves dependence on these genes for susceptibility. The findings open a door to mechanistic understanding of the role <i>SWEET</i> genes play in susceptibility and illustrate the importance of complete genome sequence-based monitoring of <i>Xoo</i> populations in developing varieties with effective disease resistance.

Item Type: Article
Uncontrolled Keywords: bacterial blight of rice, SMRT sequencing, transcription activator-like effectors (TALES), susceptibility genes, SWEET genes
Depositing User: Symplectic Admin
Date Deposited: 06 Feb 2019 10:35
Last Modified: 13 Oct 2023 20:13
DOI: 10.3389/fmicb.2018.02703
Open Access URL: https://doi.org/10.3389/fmicb.2018.02703
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3032351
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