Multiple communication mechanisms between sensor kinases are crucial for virulence in Pseudomonas aeruginosa


Francis, Vanessa I, Waters, Elaine M, Finton-James, Sutharsan E, Gori, Andrea, Kadioglu, Aras ORCID: 0000-0003-1137-6321, Brown, Alan R and Porter, Steven L (2018) Multiple communication mechanisms between sensor kinases are crucial for virulence in Pseudomonas aeruginosa NATURE COMMUNICATIONS, 9 (1). 2219-. ISSN 2041-1723, 2041-1723

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Abstract

Bacteria and many non-metazoan Eukaryotes respond to stresses and threats using two-component systems (TCSs) comprising sensor kinases (SKs) and response regulators (RRs). Multikinase networks, where multiple SKs work together, detect and integrate different signals to control important lifestyle decisions such as sporulation and virulence. Here, we study interactions between two SKs from Pseudomonas aeruginosa, GacS and RetS, which control the switch between acute and chronic virulence. We demonstrate three mechanisms by which RetS attenuates GacS signalling: RetS takes phosphoryl groups from GacS-P; RetS has transmitter phosphatase activity against the receiver domain of GacS-P; and RetS inhibits GacS autophosphorylation. These mechanisms play important roles in vivo and during infection, and exemplify an unprecedented degree of signal processing by SKs that may be exploited in other multikinase networks.

Item Type: Article
Uncontrolled Keywords: Animals, Mice, Inbred BALB C, Humans, Mice, Moths, Pseudomonas aeruginosa, Pseudomonas Infections, Disease Models, Animal, Phosphotransferases, Bacterial Proteins, Virulence Factors, Anti-Bacterial Agents, Virulence, Signal Transduction, Phosphorylation, Female, Protein Interaction Maps, Protein Domains
Depositing User: Symplectic Admin
Date Deposited: 11 Feb 2019 11:50
Last Modified: 23 May 2026 01:35
DOI: 10.1038/s41467-018-04640-8
Open Access URL: https://doi.org/10.1038/s41467-018-04640-8
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3032638
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