Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-?, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

Imrie, Heather and Williams, Diana JL ORCID: 0000-0001-8186-7236 (2019) Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-?, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity. BMC VETERINARY RESEARCH, 15 (1). 45-.

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Abstract

<h4>Background</h4>Bacterial lipopolysaccharide and interferon-γ stimulation of rodent macrophages in vitro induces up-regulation of inducible nitric oxide synthase, whereas interleukin-4 stimulation results in increased activity of arginase-1. Thus different stimulants result in differing macrophage phenotypes, appropriate for responses to a range of pathogens. The current study was conducted in order to determine whether bovine macrophages derived from monocytes and spleen respond similarly.<h4>Results</h4>Lipopolysaccharide and interferon-γ did not induce detectable increases in nitric oxide production by bovine monocyte-derived or splenic macrophages in vitro. Similarly, interleukin-4 and interleukin-13 did not affect arginase activity. However, changes in transcription of genes coding for these products were detected.<h4>Conclusion</h4>Differences between macrophage activation patterns exist between cattle and other species and these differences may occur during the post-transcription phase.

Item Type:	Article
Uncontrolled Keywords:	Cattle, Macrophage, Nitric oxide, Arginase, Inducible nitric oxide synthase
Depositing User:	Symplectic Admin
Date Deposited:	15 Feb 2019 09:37
Last Modified:	19 Jan 2023 01:03
DOI:	10.1186/s12917-019-1785-0
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3032842