Polytetrafluoroethylene tape as a low-cost hydrophobic substrate for drop-coating deposition Raman spectroscopy of proteins

Smith, James and Arnolds, Heike ORCID: 0000-0002-5723-9309 (2018) Polytetrafluoroethylene tape as a low-cost hydrophobic substrate for drop-coating deposition Raman spectroscopy of proteins. JOURNAL OF RAMAN SPECTROSCOPY, 49 (7). pp. 1236-1239.

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Official URL: http://dx.doi.org/10.1002/jrs.5371

Abstract

<jats:title>Abstract</jats:title><jats:p>Highly hydrophobic substrates are desirable for drop coating deposition Raman spectroscopy of proteins, because they concentrate the analyte during drying. Most of the commercial substrates used to date are comparatively expensive, whereas thiol or silane functionalisation of surfaces requires a degree of experience not available in a typical biology laboratory. Here, we show that polytetrafluoroethylene tape, commercially available as thread sealing tape, can be stretched across a washer to yield a highly hydrophobic surface, which is thin enough to produce only a very small Raman signal itself. We demonstrate that this simple substrate can be used to yield high‐quality spectra of biomolecules such as human insulin and lysozyme in both native and fibrillated forms.</jats:p>

Item Type:	Article
Uncontrolled Keywords:	DCDR, drop coating deposition Raman, hen egg-white lysozyme, human insulin, hydrophobic surface
Depositing User:	Symplectic Admin
Date Deposited:	25 Feb 2019 13:56
Last Modified:	18 Sep 2023 09:45
DOI:	10.1002/jrs.5371
Open Access URL:	https://doi.org/10.1002/jrs.5371
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3033392