Human cardiac mesenchymal stem cell like cells, a novel cell population with therapeutic potential.



Oldershaw, Rachel, Owens, William Andrew, Sutherland, Rachel, Linney, Martin, Liddle, Rachel, Magana, Lissette, Lash, Gendie Elizabeth, Gill, Jason, Richardson, Gavin and Meeson, Annette
(2019) Human cardiac mesenchymal stem cell like cells, a novel cell population with therapeutic potential. Stem cells and development, 28 (9). 593 - 607.

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Abstract

Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements in heart function following treatment have been reported to be variable, with some showing no response. This discrepancy in response remains unresolved. MSCs have been highlighted as a regenerative tool as these cells display both immunomodulatory and pro-regenerative activity. The purpose of this study was to derive a cardiac MSC population to provide an alternative/support to current therapies. We derived human cardiac-mesenchymal-stem-cell-like-cells (CMSCLC) so named as they share some MSC characteristics. However, CMSCLC lack the MSC tri-lineage differentiation capacity, being capable of only rare adipogenic differentiation and demonstrating low/no osteogenic or chondrogenic potential, a phenotype that may have advantages following transplantation. Further, CMSCLC expressed low levels of p16, high levels of MHCI and low levels of MHCII. A lack of senescent cells would also be advantageous for cells to be used therapeutically, as would the ability to modulate the immune response. Crucially, CMSCLC display a transcriptional profile which includes genes associated with cardio-protective/cardio-beneficial effects. CMSCLC are also secretory and multipotent, giving rise to cardiomyocytes and endothelial cells. Our findings support CMSCLC as a novel cell population suitable for use for transplantation.

Item Type: Article
Uncontrolled Keywords: cardiac stem cells, cardiosphere-cardiac-derived cells, single cell
Depositing User: Symplectic Admin
Date Deposited: 26 Mar 2019 13:57
Last Modified: 23 Feb 2021 18:11
DOI: 10.1089/scd.2018.0170
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3034582