Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.



Szatkowska, Roza, Garcia-Albornoz, Manuel, Roszkowska, Katarzyna, Holman, Stephen W, Furmanek, Emil, Hubbard, Simon J, Beynon, Robert J ORCID: 0000-0003-0857-495X and Adamczyk, Malgorzata
(2019) Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1. BIOCHEMICAL JOURNAL, 476. 1053 - 1082.

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Abstract

Protein biosynthesis is energetically costly, is tightly regulated and is coupled to stress conditions including glucose deprivation. RNA polymerase III (RNAP III)-driven transcription of tDNA genes for production of tRNAs is a key element in efficient protein biosynthesis. Here we present an analysis of the effects of altered RNAP III activity on the Saccharomyces cerevisiae proteome and metabolism under glucose-rich conditions. We show for the first time that RNAP III is tightly coupled to the glycolytic system at the molecular systems level. Decreased RNAP III activity or the absence of the RNAP III negative regulator, Maf1 elicit broad changes in the abundance profiles of enzymes engaged in fundamental metabolism in S. cerevisiae In a mutant compromised in RNAP III activity, there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux

Item Type: Article
Uncontrolled Keywords: RNA polymerase III, amino acid metabolism, comparative proteomics, glycolysis, maf1
Depositing User: Symplectic Admin
Date Deposited: 08 Apr 2019 10:01
Last Modified: 16 Apr 2021 11:21
DOI: 10.1042/BCJ20180701
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3036068