Line-Field Optical Coherence Tomography as a tool for In vitro characterization of corneal biomechanics under physiological pressures

Kazaili, Ahmed ORCID: 0000-0002-0400-7432, Lawman, Samuel ORCID: 0000-0003-2695-4366, Geraghty, Brendan ORCID: 0000-0003-0561-6667, Eliasy, A ORCID: 0000-0002-4473-1900, Zheng, Yalin ORCID: 0000-0002-7873-0922, Shen, Yaochun ORCID: 0000-0002-8915-1993 and Akhtar, Riaz ORCID: 0000-0002-7963-6874 (2019) Line-Field Optical Coherence Tomography as a tool for In vitro characterization of corneal biomechanics under physiological pressures Scientific Reports, 9 (1). 6321-. ISSN 2045-2322, 2045-2322

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Abstract

There has been a lot of interest in accurately characterising corneal biomechanical properties under intraocular pressure (IOP) to help better understand ocular pathologies that are associated with elevated IOP. This study investigates the novel use of Line-Field Optical Coherence Tomography (LF-OCT) as an elastographic tool for accurately measuring mechanical properties of porcine corneas based on volumetric deformation following varying IOPs. A custom-built LF-OCT was used to measure geometrical and corneal surface displacement changes in porcine corneas under a range of IOPs, from 0–60 mmHg. Corneal thickness, elastic properties and hysteresis were calculated as a function of pressure. In addition, the effects of hydration were explored. We found that the elastic modulus increased in a linear fashion with IOP. Corneal thickness was found to reduce with IOP, decreasing 14% from 0 to 60 mmHg. Prolonged hydration in phosphate buffered saline (PBS) was found to significantly increase the elastic modulus and corneal hysteresis. Our study demonstrates that LF-OCT can be used to accurately measure the elastic properties based on volumetric deformation following physiological pressures. Furthermore, we show that prolonged hydration in PBS has a significant effect on the measured corneal properties.

Item Type:	Article
Uncontrolled Keywords:	Cornea, Animals, Swine, Tomography, Optical Coherence, Tonometry, Ocular, Intraocular Pressure, Elastic Modulus
Depositing User:	Symplectic Admin
Date Deposited:	23 Apr 2019 09:08
Last Modified:	22 Jan 2026 01:42
DOI:	10.1038/s41598-019-42789-4
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3037533
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