The impact of poly-A microsatellite heterologies in meiotic recombination



Heissl, Angelika, Betancourt, AJ ORCID: 0000-0001-9351-1413, Hermann, Philipp, Povysil, Gundula, Arbeithuber, Barbara, Futschik, Andreas, Ebner, Thomas and Tiemann-Boege, Irene
(2019) The impact of poly-A microsatellite heterologies in meiotic recombination. Life Science Alliance, 2 (2).

Access the full-text of this item by clicking on the Open Access link.

Abstract

Meiotic recombination has strong, but poorly understood effects on short tandem repeat (STR) instability. Here, we screened thousands of single recombinant products with sperm typing to characterize the role of polymorphic poly-A repeats at a human recombination hotspot in terms of hotspot activity and STR evolution. We show that the length asymmetry between heterozygous poly-A’s strongly influences the recombination outcome: a heterology of 10 A’s (9A/19A) reduces the number of crossovers and elevates the frequency of non-crossovers, complex recombination products, and long conversion tracts. Moreover, the length of the heterology also influences the STR transmission during meiotic repair with a strong and significant insertion bias for the short heterology (6A/7A) and a deletion bias for the long heterology (9A/19A). In spite of this opposing insertion-/deletion-biased gene conversion, we find that poly-A’s are enriched at human recombination hotspots that could have important consequences in hotspot activation.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 09 May 2019 08:48
Last Modified: 03 Jun 2020 21:11
DOI: 10.26508/lsa.201900364
Open Access URL: http://www.life-science-alliance.org/content/2/2/e...
Related URLs:
URI: http://livrepository.liverpool.ac.uk/id/eprint/3040310