The impact of poly-A microsatellite heterologies in meiotic recombination

Heissl, Angelika, Betancourt, AJ ORCID: 0000-0001-9351-1413, Hermann, Philipp, Povysil, Gundula, Arbeithuber, Barbara, Futschik, Andreas, Ebner, Thomas and Tiemann-Boege, Irene (2019) The impact of poly-A microsatellite heterologies in meiotic recombination Life Science Alliance, 2 (2). e201900364-. ISSN 2575-1077, 2575-1077

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Abstract

Meiotic recombination has strong, but poorly understood effects on short tandem repeat (STR) instability. Here, we screened thousands of single recombinant products with sperm typing to characterize the role of polymorphic poly-A repeats at a human recombination hotspot in terms of hotspot activity and STR evolution. We show that the length asymmetry between heterozygous poly-A’s strongly influences the recombination outcome: a heterology of 10 A’s (9A/19A) reduces the number of crossovers and elevates the frequency of non-crossovers, complex recombination products, and long conversion tracts. Moreover, the length of the heterology also influences the STR transmission during meiotic repair with a strong and significant insertion bias for the short heterology (6A/7A) and a deletion bias for the long heterology (9A/19A). In spite of this opposing insertion-/deletion-biased gene conversion, we find that poly-A’s are enriched at human recombination hotspots that could have important consequences in hotspot activation.

Item Type:	Article
Uncontrolled Keywords:	Spermatozoa, Humans, Poly A, Meiosis, Crossing Over, Genetic, Gene Conversion, Microsatellite Repeats, Genotype, Haplotypes, Heterozygote, Polymorphism, Single Nucleotide, Alleles, Tissue Donors, Male, Microsatellite Instability, Mutation Rate
Depositing User:	Symplectic Admin
Date Deposited:	09 May 2019 08:48
Last Modified:	01 Mar 2026 09:29
DOI:	10.26508/lsa.201900364
Open Access URL:	http://www.life-science-alliance.org/content/2/2/e...
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3040310
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