Kamalian, Laleh, Douglas, Oisin, Jolly, Carol E, Snoeys, Jan, Simic, Damir, Monshouwer, Mario, Williams, Dominic P 
ORCID: 0000-0002-0758-3152, Park, B Kevin ORCID: 0000-0001-8384-824X and Chadwick, Amy E ORCID: 0000-0002-7399-8655
(2019)
Acute Metabolic Switch Assay Using Glucose/Galactose Medium in HepaRG Cells to Detect Mitochondrial Toxicity.
Current protocols in toxicology, 80 (01).
e76-.
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Abstract
Using galactose instead of glucose in the culture medium of hepatoma cell lines, such as HepG2 cells, has been utilized for a decade to unmask the mitochondrial liability of chemical compounds. A modified glucose-galactose assay on HepG2 cells, reducing the experimental period for screening of mitochondrial toxicity to 2 to 4 hr, has been previously reported. HepaRG cells are one of the few cell lines that retain some of the important characteristics of human hepatocytes, offering advantages of working with a cell line, therefore, are considered an alternative for HepG2 cells in drug toxicity screening. A method is described here using HepaRG cells in an acute metabolic switch assay utilizing specific glucose/galactose media, a combined ATP-protein-LDH assay measuring three endpoints from one 96-well plate, and a criteria to label a compound as a mitochondrial toxin. © 2019 by John Wiley & Sons, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | drug‐induced liver injury, glucose/galactose, HepaRG, mitochondria |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 May 2019 10:45 |
| Last Modified: | 19 Jan 2023 00:43 |
| DOI: | 10.1002/cptx.76 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3042308 |
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