Acute Metabolic Switch Assay Using Glucose/Galactose Medium in HepaRG Cells to Detect Mitochondrial Toxicity.



Kamalian, Laleh, Douglas, Oisin, Jolly, Carol E, Snoeys, Jan, Simic, Damir, Monshouwer, Mario, Williams, Dominic P ORCID: 0000-0002-0758-3152, Park, B Kevin ORCID: 0000-0001-8384-824X and Chadwick, Amy E ORCID: 0000-0002-7399-8655
(2019) Acute Metabolic Switch Assay Using Glucose/Galactose Medium in HepaRG Cells to Detect Mitochondrial Toxicity. Current protocols in toxicology, 80 (01).

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Abstract

Using galactose instead of glucose in the culture medium of hepatoma cell lines, such as HepG2 cells, has been utilized for a decade to unmask the mitochondrial liability of chemical compounds. A modified glucose-galactose assay on HepG2 cells, reducing the experimental period for screening of mitochondrial toxicity to 2 to 4 hr, has been previously reported. HepaRG cells are one of the few cell lines that retain some of the important characteristics of human hepatocytes, offering advantages of working with a cell line, therefore, are considered an alternative for HepG2 cells in drug toxicity screening. A method is described here using HepaRG cells in an acute metabolic switch assay utilizing specific glucose/galactose media, a combined ATP-protein-LDH assay measuring three endpoints from one 96-well plate, and a criteria to label a compound as a mitochondrial toxin. © 2019 by John Wiley & Sons, Inc.

Item Type: Article
Uncontrolled Keywords: drug‐induced liver injury, glucose/galactose, HepaRG, mitochondria
Depositing User: Symplectic Admin
Date Deposited: 21 May 2019 10:45
Last Modified: 14 Jan 2021 09:11
DOI: 10.1002/cptx.76
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3042308