Plasma Polymer Coatings To Direct the Differentiation of Mouse Kidney-Derived Stem Cells into Podocyte and Proximal Tubule-like Cells



Hopp, Isabel, MacGregor, Melanie N, Doherty, Kyle ORCID: 0000-0002-7616-1295, Visalakshan, Rahul M, Vasilev, Krasimir, Williams, Rachel ORCID: 0000-0002-1954-0256 and Murray, Patricia
(2019) Plasma Polymer Coatings To Direct the Differentiation of Mouse Kidney-Derived Stem Cells into Podocyte and Proximal Tubule-like Cells. ACS Biomaterials Science and Engineering, 5 (6). 2834 - 2845.

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Abstract

Kidney disease is now recognised as a global health problem and is associated with increased morbidity and mortality, along with high economic costs. To develop new treatments for ameliorating kidney injury and preventing disease progression, there is a need for appropriate renal culture systems for screening novel drugs and investigating the cellular mechanisms underlying renal pathogenesis. There is a need for in vitro culture systems that promote the growth and differentiation of specialised renal cell types. In this work, we have used plasma polymerisation technology to generate gradients of chemical functional groups to explore whether specific concentrations of these functional groups can direct the differentiation of mouse kidney-derived stem cells into specialised renal cell types. We found that amine-rich (-NH2) allylamine-based plasma polymerised coatings could promote differentiation into podocyte-like cells, whereas methyl-rich (CH3) 1,7-octadiene-based coatings promoted differentiation into proximal tubule-like cell (PTC). Importantly, the PT-like cells generated on the substrates expressed the marker megalin and were able to endocytose albumin, indicating that the cells were functional.

Item Type: Article
Uncontrolled Keywords: allylamine, differentiation, kidney disease, megalin, podocyte, proximal tubule cells, renal, stem cells
Depositing User: Symplectic Admin
Date Deposited: 08 Jul 2019 13:56
Last Modified: 21 Jun 2021 15:10
DOI: 10.1021/acsbiomaterials.9b00299
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3042774

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