Carissimo, Guillaume, Teo, Teck-Hui, Chan, Yi-Hao, Lee, Cheryl Yi-Pin, Lee, Bernett, Torres-Ruesta, Anthony, Tan, Jeslin JL, Chua, Tze-Kwang, Fong, Siew-Wai, Lum, Fok-Moon et al (show 1 more authors)
(2019)
<i>Viperin</i> controls chikungunya virus-specific pathogenic T cell IFNγ Th1 stimulation in mice.
LIFE SCIENCE ALLIANCE, 2 (1).
e201900298-.
Abstract
Chikungunya virus (CHIKV) has been a worldwide threat since its reemergence in La Reunion Island in 2004. Expression of the interferon-stimulated protein <i>Viperin</i> correlates with viral load burden in patients, and studies in mice have demonstrated its role to limit disease severity against CHIKV infection. Using <i>Viperin</i> <sup><i>-/-</i></sup> mice, we aimed to understand the contribution of <i>Viperin</i> to the T-cell immune response against CHIKV. CD4 T-cell depletion in <i>Viperin</i> <sup><i>-/-</i></sup> mice showed that increased late acute joint inflammation (5-8 d postinfection) was exclusively mediated by T cells. Specifically, CHIKV-infected <i>Viperin</i> <sup><i>-/-</i></sup> mice showed an increased INFγ Th1 profile of CD4 T cells, enhanced INFγ stimulation by APCs, an increased INFγ secretion profile in the joint microenvironment, and increased numbers of inflammatory monocytes in virus-infected joints compared with WT mice. Bone marrow grafting experiments showed that <i>Viperin</i> expression in both hematopoietic and non-hematopoietic cells is instrumental in reducing disease severity associated with a CD4 T-cell response.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Antigen-Presenting Cells, CD4-Positive T-Lymphocytes, Monocytes, Animals, Mice, Inbred C57BL, Mice, Knockout, Humans, Mice, Chikungunya virus, Arthritis, Proteins, Lymphocyte Depletion, Bone Marrow Transplantation, Viral Load, Female, Basic Helix-Loop-Helix Transcription Factors, Interferon-gamma, Gene Knockout Techniques, HEK293 Cells, Chikungunya Fever |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 24 May 2019 14:17 |
| Last Modified: | 06 Feb 2024 14:01 |
| DOI: | 10.26508/lsa.201900298 |
| Open Access URL: | https://doi.org/10.26508/lsa.201900298 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3042804 |
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