Parker, Tony, Wang, Kai-wen, Manning, Declan and Dart, C ORCID: 0000-0002-3509-8349
(2019)
Soluble adenylyl cyclase links Ca2+ entry to Ca2+/cAMP-response element binding protein (CREB) activation in vascular smooth muscle.
Scientific Reports, 9 (1).
7317-.
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Abstract
Ca2+-transcription coupling controls gene expression patterns that define vascular smooth muscle cell (VSMC) phenotype. Although not well understood this allows normally contractile VSMCs to become proliferative following vessel injury, a process essential for repair but which also contributes to vascular remodelling, atherogenesis and restenosis. Here we show that the Ca2+/HCO3−-sensitive enzyme, soluble adenylyl cyclase (sAC), links Ca2+ influx in human coronary artery smooth muscle cells (hCASMCs) to 3′,5′-cyclic adenosine monophosphate (cAMP) generation and phosphorylation of the transcription factor Ca2+/cAMP response element binding protein (CREB). Store-operated Ca2+ entry (SOCE) into hCASMCs expressing the FRET-based cAMP biosensor H187 induced a rise in cAMP that mirrored cytosolic [Ca2+]. SOCE also activated the cAMP effector, protein kinase A (PKA), as determined by the PKA reporter, AKAR4-NES, and induced phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and CREB. Transmembrane adenylyl cyclase inhibition had no effect on the SOCE-induced rise in cAMP, while sAC inhibition abolished SOCE-generated cAMP and significantly reduced SOCE-induced VASP and CREB phosphorylation. This suggests that SOCE in hCASMCs activates sAC which in turn activates the cAMP/PKA/CREB axis. sAC, which is insensitive to G-protein modulation but responsive to Ca2+, pH and ATP, may therefore act as an overlooked regulatory node in vascular Ca2+-transcription coupling.
Item Type: | Article |
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Uncontrolled Keywords: | Cardiovascular biology, Cell signalling |
Depositing User: | Symplectic Admin |
Date Deposited: | 29 May 2019 10:26 |
Last Modified: | 19 Jan 2023 00:42 |
DOI: | 10.1038/s41598-019-43821-3 |
Open Access URL: | https://doi.org/10.1038/s41598-019-43821-3 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3043516 |
Available Versions of this Item
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Soluble adenylyl cyclase links Ca2+ entry to Ca2+/cAMP-response element binding protein (CREB) activation in vascular smooth muscle. (deposited 17 May 2019 07:30)
- Soluble adenylyl cyclase links Ca2+ entry to Ca2+/cAMP-response element binding protein (CREB) activation in vascular smooth muscle. (deposited 29 May 2019 10:26) [Currently Displayed]