Alsahag, MA
(2019)
Phenotypic, transcriptomic, and proteomic comparison of clinically-derived Group A streptococcus isolates in the United Kingdom population.
PhD thesis, University of Liverpool.
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Abstract
Group A Streptococcus (GAS), also known as Streptococcus pyogenes, is an important cause of human diseases ranging from minor conditions such as pharyngitis to severe, life-threatening infections such as necrotising fasciitis. Between 2010 and 2012, a GAS outbreak (n=14 cases) caused by the emm32.2 GAS subtype occurred in Merseyside, which led to severe invasive disease with 29% case fatality. The outbreak was confined to adults and IV drug users; homelessness and alcohol abuse were identified as risk factors. We sought to investigate the genomic features of this subtype to explain its clinical phenotype. A first objective in my work was to carry out in vitro examination of the emm32.2 outbreak strain to identify whether phenotypic differences may be associated with specific genomic features hence explaining the epidemiological features of this emm subtype. A series of in vitro experiments were exploited and developed to determine whether and how emm32.2 might behave differently from other non-outbreak strains. We carried out a comparative examination of invasive emm32.2 isolates (n=14), against invasive and non-invasive isolates, i.e., emm6.0, emm89.0, and emm1.0, with respect to their capsule thickness, ... (continues)
| Item Type: | Thesis (PhD) |
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| Divisions: | Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Medicine |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 22 Aug 2019 10:06 |
| Last Modified: | 01 Sep 2024 18:43 |
| DOI: | 10.17638/03043566 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3043566 |
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