Thompson, Louise Miranda 
ORCID: 0000-0002-6542-4301
(2019)
Modelling barrier function of the villous and follicle-associated epithelium in 3D
PhD thesis, University of Liverpool.
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Abstract
The intestinal epithelium acts as a barrier that excludes external molecules from the tissues beneath. Defects in this barrier function are increasingly associated with pathological states, including inflammatory bowel disease, irritable bowel syndrome, coeliac disease, obesity and food allergies. Understanding of epithelial biology and barrier function has been limited by a lack of physiologically representative in vitro models. Identification of intestinal epithelial stem cells and recent methodological advances have allowed intestinal stem cells to be cultured so that they proliferate, differentiate and self-assemble to mimic in vivo tissue architecture, forming a continuous epithelium around a hollow central lumen, known as an enteroid. The initial aim of this study was to evaluate the extent to which mouse small intestinal enteroids simulate their parent tissue. The second intention was to investigate whether this system can be used to model barrier function of both villous epithelium and specialised areas of epithelium overlying gut-associated lymphoid tissues, where antigen sampling occurs due to the presence of rare phagocytic M cells. Enteroids were shown to contain all major epithelial cell lineages ... (continues)
| Item Type: | Thesis (PhD) |
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| Uncontrolled Keywords: | gastroenterology, small intestine, organoid, enteroid, follicle-associated epithelium, M cell, barrier function |
| Divisions: | Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences > School of Medicine |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Jun 2019 13:47 |
| Last Modified: | 31 Oct 2024 07:01 |
| DOI: | 10.17638/03043862 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3043862 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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