Lai, Hei Ming, Liu, Alan King Lun, Ng, Wai-Lung, DeFelice, John, Lee, Wing Sang, Li, Heng, Li, Wen, Ng, Ho Man, Chang, Raymond Chuen-Chung, Lin, Bin et al (show 2 more authors)
(2016)
Rationalisation and Validation of an Acrylamide-Free Procedure in Three-Dimensional Histological Imaging.
PLOS ONE, 11 (6).
e0158628-.
|
Text
Rationalisation and Validation of an Acrylamide-Free Procedure in Three-Dimensional Histological Imaging.pdf - Published version Download (10MB) | Preview |
Abstract
Three-dimensional visualization of intact tissues is now being achieved by turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to achieve optical transparency. To preserve tissue integrity, an acrylamide-based hydrogel has been proposed to embed the tissue. In this study, we examined the rationale behind the use of acrylamide in CLARITY, and presented evidence to suggest that the omission of acrylamide-hydrogel embedding in CLARITY does not alter the preservation of tissue morphology and molecular information in fixed tissues. We therefore propose a novel and simplified workflow for formaldehyde-fixed tissue clearing, which will facilitate the laboratory implementation of this technique. Furthermore, we have investigated the basic tissue clearing process in detail and have highlighted some areas for targeted improvement of technologies essential for the emerging subject of three-dimensional histology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Brain, Animals, Humans, Mice, Acrylamide, Chromatography, Liquid, Tissue Fixation, Mass Spectrometry |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 04 Jun 2019 09:08 |
| Last Modified: | 19 Jan 2023 00:41 |
| DOI: | 10.1371/journal.pone.0158628 |
| Open Access URL: | https://doi.org/10.1371/journal.pone.0158628 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3044356 |
Dimensions
Dimensions
