Koron, Roshan
(2018)
DNA signatures as a predictor of breast cancer risk
Doctor of Medicine thesis, University of Liverpool.
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Abstract
Introduction: Breast cancer is the most common cancer in the UK with around 54,900 new cases diagnosed each year and 11,400 breast cancer deaths reported in 2016. Early diagnosis of breast cancer and personalised treatment strategies lead to a more favourable outcome. Biomarkers are essential for disease management in terms of risk calculation, response to treatment and disease outcome. The aim of this study has been to assess whether two variable number tandem repeats (VNTRs) may help to modify prediction of breast cancer risk in patients with pathogenic germline variants in the BRCA1 and BRCA2 tumour suppressor genes. I therefore explored the polymorphism of the AAAG VNTR in the 5' untranslated region (UTR) of the oestrogen related receptor ? (ESRRG) gene and a 15 bp VNTR located in the internal promoter region of the MIR137 gene as both genes are implicated in breast cancer. Reporter gene analysis was performed on the ESRRG AAAG VNTR to observe the effects of varying repeat lengths on differential expression of the gene under basal conditions and following exposure to oestrogen in a luciferase reporter gene assay. VNTRs are often regulatory in nature therefore they may show insight into pathways underlying ... (continues)
| Item Type: | Thesis (Doctor of Medicine) |
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| Divisions: | Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences > School of Medicine |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 12 Jul 2019 10:36 |
| Last Modified: | 11 Feb 2025 22:52 |
| DOI: | 10.17638/03045626 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3045626 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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