Identification of Equid herpesvirus 2 in tissue-engineered equine tendon.



Wardle, Roisin, Pullman, Jane A ORCID: 0000-0002-7563-074X, Haldenby, Sam, Ressel, Lorenzo ORCID: 0000-0002-6614-1223, Pope, Marion, Clegg, Peter D ORCID: 0000-0003-0632-0032, Radford, Alan ORCID: 0000-0002-4590-1334, Stewart, James P ORCID: 0000-0002-8928-2037, Al-Saadi, Mohammed ORCID: 0000-0003-2751-862X, Dyer, Philip
et al (show 1 more authors) (2017) Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. Wellcome open research, 2. 60 - ?.

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Abstract

<b>Background:</b> Incidental findings of virus-like particles were identified following electron microscopy of tissue-engineered tendon constructs (TETC) derived from equine tenocytes. We set out to determine the nature of these particles, as there are few studies which identify virus in tendons <i>per se</i>, and their presence could have implications for tissue-engineering using allogenic grafts. <b>Methods:</b> Virus particles were identified in electron microscopy of TETCs. Virion morphology was used to initially hypothesise the virus identity.  Next generation sequencing was implemented to identify the virus. A pan herpesvirus PCR was used to validate the RNASeq findings using an independent platform. Histological analysis and biochemical analysis was undertaken on the TETCs. <b>Results:</b> Morphological features suggested the virus to be either a retrovirus or herpesvirus. Subsequent next generation sequencing mapped reads to Equid herpesvirus 2 (EHV2). Histological examination and biochemical testing for collagen content revealed no significant differences between virally affected TETCs and non-affected TETCs. An independent set of equine superficial digital flexor tendon tissue (n=10) examined using designed primers for specific EHV2 contigs identified at sequencing were negative. These data suggest that EHV is resident in some equine tendon. <b>Conclusions:</b> EHV2 was demonstrated in equine tenocytes for the first time; likely from <i>in vivo</i> infection. The presence of EHV2 could have implications to both tissue-engineering and tendinopathy.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 19 Sep 2019 07:34
Last Modified: 03 Apr 2021 11:00
DOI: 10.12688/wellcomeopenres.12176.2
URI: https://livrepository.liverpool.ac.uk/id/eprint/3049529

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