Identification of Equid herpesvirus 2 in tissue-engineered equine tendon.

Collapse authors list.
Wardle, Roisin, Pullman, Jane A ORCID: 0000-0002-7563-074X, Haldenby, Sam, Ressel, Lorenzo ORCID: 0000-0002-6614-1223, Pope, Marion, Clegg, Peter D ORCID: 0000-0003-0632-0032, Radford, Alan ORCID: 0000-0002-4590-1334, Stewart, James P ORCID: 0000-0002-8928-2037, Al-Saadi, Mohammed ORCID: 0000-0003-2751-862X, Dyer, Philip et al (show 1 more authors) and Peffers, Mandy J ORCID: 0000-0001-6979-0440 (2017) Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. Wellcome open research, 2. 60-.

	Text 12176_-_Mandy_Peffers (1).pdf - Published version Available under License : See the attached licence file. Download (2MB)
	Text dffb8b74-915d-4f87-8667-61d74df88b62_12176_-_mandy_peffers_v2.pdf - Published version Available under License : See the attached licence file. Download (2MB)
	Text Identification of Equid herpesvirus 2 in tissue-engineered equine tendon.pdf - Published version Available under License : See the attached licence file. Download (2MB)

Abstract

<b>Background:</b> Incidental findings of virus-like particles were identified following electron microscopy of tissue-engineered tendon constructs (TETC) derived from equine tenocytes. We set out to determine the nature of these particles, as there are few studies which identify virus in tendons <i>per se</i>, and their presence could have implications for tissue-engineering using allogenic grafts. <b>Methods:</b> Virus particles were identified in electron microscopy of TETCs. Virion morphology was used to initially hypothesise the virus identity.  Next generation sequencing was implemented to identify the virus. A pan herpesvirus PCR was used to validate the RNASeq findings using an independent platform. Histological analysis and biochemical analysis was undertaken on the TETCs. <b>Results:</b> Morphological features suggested the virus to be either a retrovirus or herpesvirus. Subsequent next generation sequencing mapped reads to Equid herpesvirus 2 (EHV2). Histological examination and biochemical testing for collagen content revealed no significant differences between virally affected TETCs and non-affected TETCs. An independent set of equine superficial digital flexor tendon tissue (n=10) examined using designed primers for specific EHV2 contigs identified at sequencing were negative. These data suggest that EHV is resident in some equine tendon. <b>Conclusions:</b> EHV2 was demonstrated in equine tenocytes for the first time; likely from <i>in vivo</i> infection. The presence of EHV2 could have implications to both tissue-engineering and tendinopathy.

Item Type:	Article
Uncontrolled Keywords:	Equine herpesvirus 2, equine, next-generation sequencing, superficial digital flexor tendon, tissue-engineered tendon
Depositing User:	Symplectic Admin
Date Deposited:	19 Sep 2019 07:34
Last Modified:	19 Jan 2023 00:37
DOI:	10.12688/wellcomeopenres.12176.2
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3049529

Available Versions of this Item

• Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. (deposited 13 Mar 2019 16:02)
  • Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. (deposited 11 Jul 2019 14:02)
    • Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. (deposited 19 Sep 2019 07:34) [Currently Displayed]