Identification of Equid herpesvirus 2 in tissue-engineered equine tendon

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Peffers, MJ ORCID: 0000-0001-6979-0440, Wardle, R, Pullman, JA ORCID: 0000-0002-7563-074X, Haldenby, S, Ressel, L ORCID: 0000-0002-6614-1223, Pope, M, Clegg, PD, Radford, A ORCID: 0000-0002-4590-1334, Stewart, JP ORCID: 0000-0002-8928-2037, Al-Saadi, M ORCID: 0000-0003-2751-862X et al (show 1 more authors) and Dyer, P (2017) Identification of Equid herpesvirus 2 in tissue-engineered equine tendon Wellcome Open Research, 2. 60-. ISSN 2398-502X, 2398-502X

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Abstract

Background: Incidental findings of virus-like particles were identified following electron microscopy of tissue-engineered tendon constructs (TETC) derived from equine tenocytes. We set out to determine the nature of these particles, as there are few studies which identify virus in tendons per se, and their presence could have implications for tissue-engineering using allogenic grafts. Methods: Virus particles were identified in electron microscopy of TETCs. Virion morphology was used to initially hypothesise the virus identity. Next generation sequencing was implemented to identify the virus. A pan herpesvirus PCR was used to validate the RNASeq findings using an independent platform. Histological analysis and biochemical analysis was undertaken on the TETCs. Results: Morphological features suggested the virus to be either a retrovirus or herpesvirus. Subsequent next generation sequencing mapped reads to Equid herpesvirus 2 (EHV2). Histological examination and biochemical testing for collagen content revealed no significant differences between virally affected TETCs and non-affected TETCs. An independent set of equine superficial digital flexor tendon tissue (n=10) examined using designed primers for specific EHV2 contigs identified at sequencing were negative. These data suggest that EHV is resident in some equine tendon. Conclusions: EHV2 was demonstrated in equine tenocytes for the first time; likely from in vivo infection. The presence of EHV2 could have implications to both tissue-engineering and tendinopathy.

Item Type:	Article
Uncontrolled Keywords:	Equine herpesvirus 2, equine, next-generation sequencing, superficial digital flexor tendon, tissue-engineered tendon
Depositing User:	Symplectic Admin
Date Deposited:	19 Sep 2019 07:34
Last Modified:	23 May 2026 01:43
DOI:	10.12688/wellcomeopenres.12176.2
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3049529
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Available Versions of this Item

• Identification of Equid herpesvirus 2 in tissue-engineered equine tendon (deposited 13 Mar 2019 16:02)
  • Identification of Equid herpesvirus 2 in tissue-engineered equine tendon (deposited 11 Jul 2019 14:02)
    • Identification of Equid herpesvirus 2 in tissue-engineered equine tendon (deposited 19 Sep 2019 07:34) [Currently Displayed]