Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008-2017))



Isgren, CM, Edwards, T, Pinchbeck, GL ORCID: 0000-0002-5671-8623, Winward, E, Adams, ER, Norton, P, Timofte, D ORCID: 0000-0002-7261-738X, Maddox, TW, Clegg, PD ORCID: 0000-0003-0632-0032 and Williams, NJ
(2019) Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008-2017)). BMC VETERINARY RESEARCH, 15.

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Abstract

Background This study investigated changes over time in the epidemiology of extended-spectrum β-lactamase (ESBL) producing Escherichia coli within a single equine referral hospital in the UK. Faecal samples were collected from hospitalised horses in 2008 and 2017, processed using selective media and standard susceptibility laboratory methods. A novel real-time PCR with high resolution melt analysis was used to distinguish blaCTX-M-1 and blaCTX-M-15 within CTX-M-1 group. Results In 2008, 457 faecal samples from 103 horses were collected, with ESBL-producing E. coli identified in 131 samples (28.7, 95% CI 24.6–33.1). In 2017, 314 faecal samples were collected from 74 horses with ESBL-producing E. coli identified in 157 samples (50.0, 95% CI 44.5–55.5). There were 135 and 187 non-duplicate ESBL-producing isolates from 2008 and 2017, respectively. In 2008, 12.6% of isolates belonged to CTX-M-1 group, all carrying blaCTX-M-1, whilst in 2017, 94.1% of isolates were CTX-M-1 group positive and of these 39.2 and 60.8% of isolates carried blaCTX-M-1 and blaCTX-M-15, respectively. In addition, the prevalence of doxycycline, gentamicin and 3rd generation cephalosporin resistance increased significantly from 2008 to 2017 while a decreased prevalence of phenotypic resistance to potentiated sulphonamides was observed. Conclusions The real-time PCR proved a reliable and high throughput method to distinguish between blaCTX-M-1 and blaCTX-M-15. Furthermore, its use in this study demonstrated the emergence of faecal carriage of CTX-M-15 in hospitalised horses, with an increase in prevalence of ESBL-producing E. coli as well as increased antimicrobial resistance to frequently used antimicrobials.

Item Type: Article
Uncontrolled Keywords: ESBL-producing E, coli, Melt curve analysis, Multidrug resistance, High resolution melt, Real time PCR, CTX-M-1 group
Depositing User: Symplectic Admin
Date Deposited: 05 Aug 2019 15:14
Last Modified: 11 Jun 2021 23:12
DOI: 10.1186/s12917-019-2011-9
Open Access URL: https://bmcvetres.biomedcentral.com/articles/10.11...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3051043