CD166(high) Uveal Melanoma Cells Represent a Subpopulation With Enhanced Migratory Capacity

Djirackor, Luna, Kalirai, Helen ORCID: 0000-0002-4440-2576, Coupland, Sarah E ORCID: 0000-0002-1464-2069 and Petrovski, Goran
(2019) CD166(high) Uveal Melanoma Cells Represent a Subpopulation With Enhanced Migratory Capacity. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 60 (7). pp. 2696-2704.

Access the full-text of this item by clicking on the Open Access link.
[img] Text
i1552-5783-60-7-2696.pdf - Published version

Download (1MB) | Preview


<h4>Purpose</h4>Cancer stem cells (CSCs) are a subpopulation of cells with the capacity to drive tumor growth. While there is evidence of the existence of CSCs in uveal melanoma (UM), there is no consensus on their defining markers. In this study, we examined putative CSC markers in UM cell lines, primary UM (PUM), and normal choroidal melanocytes (NCM).<h4>Methods</h4>Nonadherent sphere assays were used to assess the tumorigenic potential of 15 PUMs, 8 high (M3) and 7 low (D3) metastatic risk. Flow cytometry was used to compare the expression of CSC markers between 10 PUMs and 4 NCMs, as well as in 8 UM cell lines grown under adherent and nonadherent conditions. Based on the data generated and from TCGA analyses, CD166 was investigated in detail, including its effect on cell migration using a tumor transendothelial migration assay.<h4>Results</h4>M3 PUM had a greater melanosphere-forming efficiency than D3 PUM. CD166 and Nestin expression was upregulated in PUM compared to NCM by flow cytometry. UM cell lines resistant to anoikis had increased levels of CD271, Nestin, and CD166 compared with adherent cells. TCGA analysis showed that patients with higher CD166 expression had a poorer prognosis: this was supported by a Mel270 CD166high subpopulation that had enhanced migratory capabilities compared with CD166low cells. IHC showed that CD166 is expressed in the cytoplasm and cell membrane of PUM cells.<h4>Conclusions</h4>UM contain a population of cells with characteristics of CSCs. In particular, CD166high UM cells appear to represent a subpopulation with enhanced migratory capacity.

Item Type: Article
Uncontrolled Keywords: uveal melanoma, cancer stem cells, CD166
Depositing User: Symplectic Admin
Date Deposited: 08 Aug 2019 09:22
Last Modified: 19 Jan 2023 00:35
DOI: 10.1167/iovs.18-26431
Open Access URL:
Related URLs: