Chicken Organic Anion-Transporting Polypeptide 1A2, a Novel Avian Hepatitis E Virus (HEV) ORF2-Interacting Protein, Is Involved in Avian HEV Infection



Li, Huixia, Fan, Mengnan, Liu, Baoyuan, Ji, Pinpin, Chen, Yiyang, Zhang, Beibei, Sun, Yani, Huang, Baicheng, Nan, Yuchen, Sun, Zhenzhao
et al (show 4 more authors) (2019) Chicken Organic Anion-Transporting Polypeptide 1A2, a Novel Avian Hepatitis E Virus (HEV) ORF2-Interacting Protein, Is Involved in Avian HEV Infection. JOURNAL OF VIROLOGY, 93 (11). e02205-e02218.

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Abstract

Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease in chickens. Due to the absence of a highly effective cell culture system, there are few reports about the interaction between avian HEV and host cells. In this study, organic anion-transporting polypeptide 1A2 (OATP1A2) from chicken liver cells was identified to interact with ap237, a truncated avian HEV capsid protein spanning amino acids 313 to 549, by a glutathione S-transferase (GST) pulldown assay. GST pulldown and indirect enzyme-linked immunosorbent assays (ELISAs) further confirmed that the extracellular domain of OATP1A2 directly binds with ap237. The expression levels of OATP1A2 in host cells are positively correlated with the amounts of ap237 attachment and virus infection. The distribution of OATP1A2 in different tissues is consistent with avian HEV infection in vivo. Finally, when the functions of OATP1A2 in cells are inhibited by its substrates or an inhibitor or blocked by ap237 or anti-OATP1A2 sera, attachment to and infection of host cells by avian HEV are significantly reduced. Collectively, these results displayed for the first time that OATP1A2 interacts with the avian HEV capsid protein and can influence viral infection in host cells. The present study provides new insight to understand the process of avian HEV infection of host cells.

Item Type: Article
Uncontrolled Keywords: ORF2, avian HEV, hepatitis E virus, organic anion-transporting polypeptide
Depositing User: Symplectic Admin
Date Deposited: 13 Aug 2019 12:30
Last Modified: 19 Jan 2023 00:31
DOI: 10.1128/JVI.02205-18
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3051621