Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth <i>Brucella</i> sp. BCCN84.3



Guzman-Verri, Caterina, Suarez-Esquivel, Marcela, Ruiz-Villalobos, Nazareth, Zygmunt, Michel S, Gonnet, Mathieu, Campos, Elena, Viquez-Ruiz, Eunice, Chacon-Diaz, Carlos, Aragon-Aranda, Beatriz, Conde-Alvarez, Raquel
et al (show 7 more authors) (2019) Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth <i>Brucella</i> sp. BCCN84.3. FRONTIERS IN VETERINARY SCIENCE, 6 (JUN). 175-.

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Abstract

Members of the genus <i>Brucella</i> cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of <i>Brucella</i> species that cause disease in mammals, including humans. A <i>Brucella</i> species, labeled as <i>Brucella</i> sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as "<i>Brucella melitensis</i> biovar 2." Further molecular typing, identified the strain as an atypical "<i>Brucella suis</i>." Distinctive <i>Brucella</i> sp. BCCN84.3 markers, absent in other <i>Brucella</i> species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and <i>omp25</i> and <i>omp2a/omp2b</i> gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described <i>Brucella</i> species. The smooth <i>Brucella</i> sp. BCCN84.3 clusters together with the classical <i>Brucella</i> clade and displays all the genes required for virulence. <i>Brucella</i> sp. BCCN84.3 is a <i>species nova</i> taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the <i>Brucella</i> species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.

Item Type: Article
Uncontrolled Keywords: Brucella, Brucella melitensis, Brucella suis, Brucella canis, brucellosis, dog, species, epididymitis
Depositing User: Symplectic Admin
Date Deposited: 14 Aug 2019 13:28
Last Modified: 14 Oct 2023 01:40
DOI: 10.3389/fvets.2019.00175
Open Access URL: https://doi.org/10.3389/fvets.2019.00175
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3051737