Parkinson’s disease: a systemic inflammatory disease accompanied by bacterial inflammagens

Adams, Büin, Nunes, Massimo, Page, Martin, Roberts, Timothy ORCID: 0000-0002-1464-0151, Carr, Jonathan, Nell, Theo, Kell, Douglas ORCID: 0000-0001-5838-7963 and Pretorius, Etheresia (2019) Parkinson’s disease: a systemic inflammatory disease accompanied by bacterial inflammagens. Frontiers in Aging Neuroscience, 11 (JUL). 210-. ISSN 1663-4365, 1663-4365

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Official URL: https://doi.org/10.1101/646307

Abstract

ABSTRACT Parkinson’s disease (PD) is a well-known neurodegenerative disease. Recently, the role of gingipains from Porphyromonas gingivalis was implicated in Alzheimer’s disease. Here we present evidence of systemic inflammation, accompanied by hypercoagulation; we also show that ginipains from P. gingivalis and its LPS may foster abnormal clotting, and that ginipains are present in PD blood, and thus that ginipain’s action on blood may be relevant to PD pathology. Bloods from both PD and healthy blood samples were analysed using thromboelastography (TEG), confocal and electron microscopies, and for cytokine and other circulating biomarkers. We also probed PD and healthy plasma clots with a polyclonal antibody for the bacterial protease, gingipain R1, from P. gingivalis . Low concentrations of recombinant gingipain R1 were also added to purified fluorescent fibrinogen. TEG, fibrin(ogen) amyloid formation and platelet ultrastructure analysis confirmed profound hypercoagulation, while the biomarker analysis confirmed significantly increased levels of circulating proinflammatory cytokines. We provide evidence for the presence of the protease, gingipain R1 in PD blood, implicating inflammatory microbial cell wall products in PD.

Item Type:	Article
Uncontrolled Keywords:	Parkinson's disease, systemic inflammation, cytokines, LPS from Porphyromonas gingivalis, gingipains, amyloid formation
Depositing User:	Symplectic Admin
Date Deposited:	28 Aug 2019 10:42
Last Modified:	07 Dec 2024 19:42
DOI:	10.3389/fnagi.2019.00210
Open Access URL:	https://doi.org/10.3389/fnagi.2019.00210
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3052707