Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism



Cuskin, Fiona, Lowe, Elisabeth C, Temple, Max J, Zhu, Yanping, Cameron, Elizabeth A, Pudlo, Nicholas A, Porter, Nathan T, Urs, Karthik, Thompson, Andrew J, Cartmell, Alan ORCID: 0000-0002-5512-249X
et al (show 22 more authors) (2015) Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. NATURE, 517 (7533). 165 - U86.

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Abstract

Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a ‘selfish’ model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 10 Sep 2019 15:38
Last Modified: 19 May 2022 18:10
DOI: 10.1038/nature13995
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3054148