Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism

Cuskin, Fiona, Lowe, Elisabeth C, Temple, Max J, Zhu, Yanping, Cameron, Elizabeth A, Pudlo, Nicholas A, Porter, Nathan T, Urs, Karthik, Thompson, Andrew J, Cartmell, Alan ORCID: 0000-0002-5512-249X
et al (show 22 more authors) (2015) Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. NATURE, 517 (7533). 165-U86.

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Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a ‘selfish’ model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

Item Type: Article
Uncontrolled Keywords: Gastrointestinal Tract, Periplasm, Animals, Humans, Mice, Bacteroidetes, Yeasts, Enzymes, Glycoproteins, Mannose, Mannans, Oligosaccharides, Diet, Germ-Free Life, Carbohydrate Conformation, Models, Biological, Models, Molecular, Female, Male, Genetic Loci, Biological Evolution
Depositing User: Symplectic Admin
Date Deposited: 10 Sep 2019 15:38
Last Modified: 19 Jan 2023 00:27
DOI: 10.1038/nature13995
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