Antimicrobial nitric oxide releasing contact lens gels for the treatment of microbial keratitis.



Aveyard, Jenny Louise, Deller, Robert C ORCID: 0000-0002-5812-583X, Lace, Rebecca ORCID: 0000-0001-9410-9506, Williams, Rachel L ORCID: 0000-0002-1954-0256, Kaye, Stephen B ORCID: 0000-0003-0390-0592, Kolegraff, Keli N, Curran, Judith ORCID: 0000-0003-1551-2917 and D'Sa, Raechelle A ORCID: 0000-0003-2651-8783
(2019) Antimicrobial nitric oxide releasing contact lens gels for the treatment of microbial keratitis. ACS applied materials & interfaces.

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Abstract

Microbial keratitis is a serious sight threatening infection affecting approximately two million individuals worldwide annually. Whilst antibiotic eye drops remain the gold standard treatment for these infections, the significant problems associated with eye drop drug delivery and the alarming rise in antimicrobial resistance has meant that there is an urgent need to develop alternative treatments. In this work, a nitric oxide releasing contact lens gel displaying broad spectrum antimicrobial activity against two of the most common causative pathogens of bacterial keratitis is described. The contact lens gel is comprised of poly-ɛ-lysine (pɛK) functionalised with nitric oxide (NO) releasing diazeniumdiolate moieties which enables the controlled and sustained release of bactericidal concentrations of NO at physiological pH over a period of 15 hrs. Diazeniumdiolate functionalisation was confirmed by Fourier transform infrared (FTIR) and UV Vis spectroscopy and the concentration of NO released from the gels was determined by chemiluminescence. The bactericidal efficacy of the gels against Pseudomonas aeruginosa and Staphylococcus aureus was ascertained and between 1 and 4 log reductions in bacterial populations were observed over 24 hrs. Additional cell cytotoxicity studies with human corneal epithelial cells (HCE-T) also demonstrated that the contact lens gels were not cytotoxic suggesting that the developed technology could be a viable alternative treatment for bacterial keratitis.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 26 Sep 2019 10:29
Last Modified: 17 Sep 2021 16:10
DOI: 10.1021/acsami.9b13958
URI: https://livrepository.liverpool.ac.uk/id/eprint/3055973