Nwikue, GB
(2019)
Investigation into the mechanisms of Stevens-Johnson syndrome and toxic epidermal necrolysis
PhD thesis, University of Liverpool.
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Abstract
Stevens-Johnson syndrome and toxic epidermal necrolysis (SJS/TEN) are life-threatening immune-mediated blistering cutaneous reactions with mortality rates ranging from 10-30%. The pathophysiological mechanisms of SJS/TEN are complex and not fully understood. However, fulminant keratinocyte death and inflammation have been suggested as the major players in the mechanisms of disease. To further understand the mechanisms of SJS/TEN, we utilised both clinical samples and in vitro human immortalised keratinocyte cell line (HaCaT) cell death models to investigate mediators and putative biomarkers of SJS/TEN. HaCaT cells are phenotypically similar to primary keratinocytes and represent a suitable in vitro model for dermatologic studies. A previous study had suggested that serum profiles of high mobility group box-1 (HMGB1) protein, a known of marker of cell death and inflammation distinguished SJS/TEN from other cutaneous phenotypes (drug rash with eosinophilia and systemic symptoms, DRESS and maculopapular exanthema, MPE). To further elucidate the putative role of HMGB1 in SJS/TEN, serum, blister-fluid and skin biopsy samples from SJS/TEN were analysed for HMGB1 profile and expression. HMGB1 levels in serum were ... (continues)
| Item Type: | Thesis (PhD) |
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| Divisions: | Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences > School of Medicine |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 Jan 2020 11:35 |
| Last Modified: | 07 Feb 2025 13:30 |
| DOI: | 10.17638/03056025 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3056025 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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