Covalent Aurora A regulation by the metabolic integrator coenzyme A

Tsuchiya, Yugo, Byrne, Dominic P, Burgess, Selena G, Bormann, Jenny, Bakovic, Jovana, Huang, Yueyang, Zhyvoloup, Alexander, Yu, Bess Yi Kun, Peak-Chew, Sew, Tran, Trang
et al (show 14 more authors) (2020) Covalent Aurora A regulation by the metabolic integrator coenzyme A. REDOX BIOLOGY, 28. 101318-.

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Aurora A kinase is a master mitotic regulator whose functions are controlled by several regulatory interactions and post-translational modifications. It is frequently dysregulated in cancer, making Aurora A inhibition a very attractive antitumor target. However, recently uncovered links between Aurora A, cellular metabolism and redox regulation are not well understood. In this study, we report a novel mechanism of Aurora A regulation in the cellular response to oxidative stress through CoAlation. A combination of biochemical, biophysical, crystallographic and cell biology approaches revealed a new and, to our knowledge, unique mode of Aurora A inhibition by CoA, involving selective binding of the ADP moiety of CoA to the ATP binding pocket and covalent modification of Cys290 in the activation loop by the thiol group of the pantetheine tail. We provide evidence that covalent CoA modification (CoAlation) of Aurora A is specific, and that it can be induced by oxidative stress in human cells. Oxidising agents, such as diamide, hydrogen peroxide and menadione were found to induce Thr 288 phosphorylation and DTT-dependent dimerization of Aurora A. Moreover, microinjection of CoA into fertilized mouse embryos disrupts bipolar spindle formation and the alignment of chromosomes, consistent with Aurora A inhibition. Altogether, our data reveal CoA as a new, rather selective, inhibitor of Aurora A, which locks this kinase in an inactive state via a "dual anchor" mechanism of inhibition that might also operate in cellular response to oxidative stress. Finally and most importantly, we believe that these novel findings provide a new rationale for developing effective and irreversible inhibitors of Aurora A, and perhaps other protein kinases containing appropriately conserved Cys residues.

Item Type: Article
Uncontrolled Keywords: Animals, Humans, Mice, Coenzyme A, Crystallography, X-Ray, Protein Conformation, Oxidative Stress, Phosphorylation, Models, Molecular, Hep G2 Cells, HEK293 Cells, Spindle Apparatus, Aurora Kinase A
Depositing User: Symplectic Admin
Date Deposited: 02 Oct 2019 09:57
Last Modified: 19 Jan 2023 00:24
DOI: 10.1016/j.redox.2019.101318
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