The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model

Fiorentzis, Miltiadis, Viestenz, Arne, Siebolts, Udo, Seitz, Berthold, Coupland, Sarah E ORCID: 0000-0002-1464-2069 and Heinzelmann, Joana (2019) The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model. CANCERS, 11 (9). E1344-.

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Official URL: http://dx.doi.org/10.3390/cancers11091344

Abstract

Uveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver, bone metastases, and unresectable pancreas lesions. The aim of this study was to evaluate the effect of ECT in vitro in 3D spheroid culture systems in primary and metastatic UM cell lines. We also investigated the chick embryo chorioallantoic membrane (CAM) as an in vivo model system for the growth and treatment of UM tumors using ECT. The cytotoxic effect of ECT in 3D spheroids was analyzed seven days following treatment by assessment of the size and MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction] assay. The cytotoxicity of ECT after intratumoral or intraarterial administration was evaluated histologically. In vitro and in vivo ECT caused a significant reduction in tumor size and viability compared to electroporation or chemotherapy in both sections of our study. The current results underline the effectiveness of ECT in the treatment of UM and prepare the way for further investigation of its potential application in UM.

Item Type:	Article
Uncontrolled Keywords:	electrochemotherapy, uveal melanoma, tumor spheroids, chick embryo chorioallantoic membrane, treatment, bleomycin, electroporation, in vitro, in vivo
Depositing User:	Symplectic Admin
Date Deposited:	03 Oct 2019 09:05
Last Modified:	19 Jan 2023 00:24
DOI:	10.3390/cancers11091344
Open Access URL:	https://doi.org/10.3390/cancers11091344
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3056850